Immature Monocyte-Derived Dendritic Cells Are Productively Infected with Herpes Simplex Virus Type 1
| Autor: | Lidija Bosnjak, Anthony L. Cunningham, Zorka Mikloska |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Immunology
Herpesvirus 1 Human Virus Replication medicine.disease_cause Major histocompatibility complex Microbiology Monocytes Virus Immature Monocyte Antigens CD1 Immune system Virology medicine Humans Antigens Viral CD86 CD40 biology Dendritic Cells Intercellular Adhesion Molecule-1 Herpes simplex virus Insect Science biology.protein Pathogenesis and Immunity CD80 |
| Zdroj: | Journal of Virology. 75:5958-5964 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.75.13.5958-5964.2001 |
| Popis: | Herpes simplex viruses (HSV) have developed several immunoevasive strategies. Here we demonstrate a novel mechanism by which HSV type 1 may interfere with the immune response through infection of immature dendritic cells (DC) and selective downmodulation of costimulatory molecules. In our study we show productive infection of immature monocyte-derived DC, which closely resemble sessile Langerhans cells, by sequential expression of immediate-early, early, and late viral proteins and of glycoprotein D mRNA, as well as production of infectious virus of moderate titers. Infection was cytopathic, with the progressive loss of 20 to 45% of cells from 24 to 48 h after infection, with no more than 80% of DC found to be infected. These results are in contrast to those of previous findings of nonpermissive or abortive infection of monocytes and mature monocyte-derived DC. Infection of immature DC also led to selective and asynchronous downregulation of CD1a, CD40, CD54 (ICAM-1) (12 h postinfection), CD80 (24 h postinfection), and CD86 (48 h postinfection) but not of CD11c or major histocompatibility complex class I and II molecules when compared to DC exposed to UV-inactivated virus. Thus, we propose that productive infection of epidermal Langerhans cells in vivo may lead to delayed activation of T cells, allowing more time for replication of HSV type 1 in epidermal cells. |
| Databáze: | OpenAIRE |
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