Psychological Stress on Female Mice Diminishes the Developmental Potential of Oocytes: A Study Using the Predatory Stress Model
| Autor: | Yi-Long Miao, Ya-Nan Cheng, Jing-He Tan, Yu-Xiang Liu, Li-Hua Zhao, De-Li Wei, Ming-Jiu Luo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Embryology Hydrocortisone lcsh:Medicine Cell Count Toxicology Social and Behavioral Sciences Eating Mice Human fertilization Endocrinology Pregnancy Psychology lcsh:Science media_common Multidisciplinary Activation Program Obstetrics and Gynecology Embryo Embryo transfer medicine.anatomical_structure Mental Health Medicine Female Research Article Ovulation medicine.medical_specialty media_common.quotation_subject Psychological Stress Embryonic Development Biology Internal medicine medicine Animals Blastocyst Equine chorionic gonadotropin Preterm Labor lcsh:R Embryogenesis Oocyte Female Subfertility Fertilization Predatory Behavior Miscarriage and Stillbirth Cats Oocytes lcsh:Q Stress Psychological Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 10, p e48083 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Although the predatory stress experimental protocol is considered more psychological than the restraint protocol, it has rarely been used to study the effect of psychological stress on reproduction. Few studies exist on the direct effect of psychological stress to a female on developmental competence of her oocytes, and the direct effect of predatory maternal stress on oocytes has not been reported. In this study, a predatory stress system was first established for mice with cats as predators. Beginning 24 h after injection of equine chorionic gonadotropin, female mice were subjected to predatory stress for 24 h. Evaluation of mouse responses showed that the predatory stress system that we established increased anxiety-like behaviors and plasma cortisol concentrations significantly and continuously while not affecting food and water intake of the mice. In vitro experiments showed that whereas oocyte maturation and Sr(2+) activation or fertilization were unaffected by maternal predatory stress, rate of blastocyst formation and number of cells per blastocyst decreased significantly in stressed mice compared to non-stressed controls. In vivo embryo development indicated that both the number of blastocysts recovered per donor mouse and the average number of young per recipient after embryo transfer of blastocysts with similar cell counts were significantly lower in stressed than in unstressed donor mice. It is concluded that the predatory stress system we established was both effective and durative to induce mouse stress responses. Furthermore, predatory stress applied during the oocyte pre-maturation stage significantly impaired oocyte developmental potential while exerting no measurable impact on nuclear maturation, suggesting that cytoplasmic maturation of mouse oocytes was more vulnerable to maternal stress than nuclear maturation. |
| Databáze: | OpenAIRE |
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