Prooxidant and antioxidant effects of ascorbate on tBuOOH-induced erythrocyte membrane damage
| Autor: | Arnold Stern, Erol Baysal, Stephen Gene Sullivan |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Antioxidant
DTNB medicine.medical_treatment Ascorbic Acid Deferoxamine In Vitro Techniques medicine.disease_cause Biochemistry Antioxidants Lipid peroxidation chemistry.chemical_compound tert-Butylhydroperoxide medicine Humans Sulfhydryl Compounds Erythrocyte Membrane Thiobarbiturates Ascorbic acid Peroxides Kinetics Red blood cell medicine.anatomical_structure Membrane chemistry Ethylmaleimide Lipid Peroxidation Oxidation-Reduction Oxidative stress medicine.drug |
| Zdroj: | International Journal of Biochemistry. 21:1109-1113 |
| ISSN: | 0020-711X |
| DOI: | 10.1016/0020-711x(89)90051-7 |
| Popis: | 1. t-Butylhydroperoxide (tBuOOH) a lipoperoxide analog, causes rapid and considerable sulphydryl (SH) oxidation but almost no lipid peroxidation in red blood cell membranes (ghosts) containing no detectable haemoglobin. 2. tBuOOH, in the presence of ascorbate, produces significant lipid peroxidation the level of which is proportional to the ascorbate concentration. The initiation of lipid peroxidation is thought to occur by the reactive tBuO (butoxyl) species via the reductive decomposition of tBuOOH by ascorbate. 3. Ascorbate protects ghost membranes from the tBuOOH-induced SH oxidation in a dose-dependent fashion. 4. There is no parallelism between lipid peroxidation and SH oxidation in these systems. This suggests that the two processes occur independently of each other. 5. These findings indicate that, simultaneously, ascorbate can have both a protective and a prooxidant action in different membrane components under the same oxidative stress. |
| Databáze: | OpenAIRE |
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