A Novel Catechol Oxidase Enzyme Electrode for the Specific Determination of Catechol
| Autor: | Erol Akyilmaz, Azmi Telefoncu, Seçil Önal, Figen Zihnioglu, Sinan Akgöl, Erhan Dinçkaya |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Catechol Chromatography Immobilized enzyme biology Chemistry Stereochemistry Organic Chemistry Enzyme electrode General Medicine Applied Microbiology and Biotechnology Biochemistry Enzyme assay Analytical Chemistry chemistry.chemical_compound Enzyme Electrode biology.protein Catechol oxidase Molecular Biology Biosensor Biotechnology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0916-8451 |
| Popis: | An enzyme electrode for the specific determination of catechol was developed by using catechol oxidase (EC 1.10.3.1) from eggplant (Solanum melangena L.) in combination with a dissolved oxygen probe. Optimization studies of the prepared catechol oxidase enzyme electrode established a phosphate buffer 50 mM at pH 7.0 and 35°C to provide the optimum conditions for affirmative electrode response. The enzyme electrode response depended linearly on a catechol concentration range of 5•10(-7)-30•10(-5) M with a response time of 25 sec and substrate specificity of the catechol oxidase electrode of 100%. The biosensor retained its enzyme activity for at least 70 days. |
| Databáze: | OpenAIRE |
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