The Drosophila Hox gene Ultrabithorax controls appendage shape by regulating extracellular matrix dynamics
| Autor: | Celia García-Cortés, José Manuel de las Heras, L. S. Shashidhara, Ernesto Sánchez-Herrero, José Carlos Pastor-Pareja, David Foronda |
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| Přispěvatelé: | Ministerio de Economía, Industria y Competitividad (España), Fundación Ramón Areces, Ministerio de Ciencia e Innovación (España) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Appendage Wing animal structures Extracellular matrix Biology Ultrabithorax Cell biology Organ shape 03 medical and health sciences Apposition Hox genes 030104 developmental biology Matrix metalloproteinases Halteres Drosophila Hox gene Molecular Biology Extracellular Matrix Degradation Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Although the specific form of an organ is frequently important for its function, the mechanisms underlying organ shape are largely unknown. In Drosophila, the wings and halteres, homologous appendages of the second and third thoracic segments, respectively, bear different forms: wings are flat, whereas halteres are globular, and yet both characteristic shapes are essential for a normal flight. The Hox gene Ultrabithorax (Ubx) governs the difference between wing and haltere development, but how Ubx function in the appendages prevents or allows flat or globular shapes is unknown. Here, we show that Ubx downregulates Matrix metalloproteinase 1 (Mmp1) expression in the haltere pouch at early pupal stage, which in turn prevents the rapid clearance of Collagen IV compared with the wing disc. This difference is instrumental in determining cell shape changes, expansion of the disc and apposition of dorsal and ventral layers, all of these phenotypic traits being characteristic of wing pouch development. Our results suggest that Ubx regulates organ shape by controlling Mmp1 expression, and the extent and timing of extracellular matrix degradation Ministerio de Ciencia e Innovación (BFU2011-26075 to E.S.-H.); the Ministerio de Economı́a, Industria y Competitividad, Gobierno de España (BFU2014-51989-P to E.S.-H.; Fundación Ramón Areces |
| Databáze: | OpenAIRE |
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