The Degree of Branching of the Glycans of α1-Acid Glycoprotein in Asthma
| Autor: | De Graaff Cs, Van Dijk W, Hoefsmit Ec, Poland Dc, Beelen Rh, Pieter E. Postmus, Van Den Heuvel Mm |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Glycan Allergy Adolescent Pulmonary Fibrosis Orosomucoid Critical Care and Intensive Care Medicine Bronchial Provocation Tests Atopy Leukocyte Count Polysaccharides Forced Expiratory Volume Respiratory Hypersensitivity medicine Humans Aged Asthma chemistry.chemical_classification biology medicine.diagnostic_test business.industry Middle Aged respiratory system medicine.disease Blood proteins respiratory tract diseases Eosinophils body regions Bronchoalveolar lavage chemistry Immunology biology.protein Glycoprotein business Bronchoalveolar Lavage Fluid Immunoelectrophoresis Two-Dimensional |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 161:1972-1978 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.161.6.9812022 |
| Popis: | Alpha(1)-acid glycoprotein (AGP) is a plasma protein belonging to the group of acute-phase proteins. It contains five N-linked glycans which, depending on pathophysiologic state, differ in their degree of branching (i.e., in the relative proportions of di-, tri-, and tetraantennary glycans). Changes in the degree of branching of these glycans have been shown to affect various immunomodulatory properties of AGP. We wanted to investigate whether changes occur in the branching of AGP glycans in plasma and in bronchoalveolar lavage fluid (BALF) in asthma. For this purpose, we selected three groups of patients for study: patients with atopic asthma (AA), atopic nonasthmatic patients, and a group of patients with various interstitial lung diseases (ILDs). The plasma AGP concentration was normal in both atopic study groups, but was increased in ILD patients. In contrast, the branching of glycans of AGP was altered in subjects with AA, whereas it was normal in the other study groups. The presence of asthma symptoms correlated with the increased glycan branching of AGP in both plasma and BALF. Additionally, the degree of branching of AGP in BALF was related to FEV(1), to the provocative dose of histamine causing a 20% decrease in FEV (PD(20)), and to the number of eosinophils. In conclusion, asthma is accompanied by changes in the branching of AGP glycans that indicate an inflammatory reaction that differs markedly from a normal acute-phase response, in which decreased branching of AGP occurs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|