THz irradiation inhibits cell division by affecting actin dynamics
| Autor: | Ryosuke Hosoki, Yuichi Ogawa, Yuya Ueno, Masahiko Harata, Chiko Otani, Toshitaka Idehara, Takanori Saito, Hiromichi Hoshina, Shota Yamazaki, Yuusuke Yamaguchi |
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| Rok vydání: | 2021 |
| Předmět: |
Cell division
Science Cell Membranes Mitosis Actin Filaments macromolecular substances 02 engineering and technology Biochemistry Filamentous actin 03 medical and health sciences Contractile Proteins Humans Cell Cycle and Cell Division Irradiation Interphase Actin Cytokinesis 030304 developmental biology 0303 health sciences Multidisciplinary Chromosome Biology Chemistry Biology and Life Sciences Proteins Cell Biology 021001 nanoscience & nanotechnology Actins Cell Motility Cytoskeletal Proteins Microscopy Fluorescence Polymerization Cell Processes Cytoplasm Biophysics Dynamic Actin Filaments Medicine Single-Cell Analysis Cellular Structures and Organelles 0210 nano-technology Cell Division Terahertz Radiation HeLa Cells Research Article Actin Polymerization |
| Zdroj: | PLoS ONE, Vol 16, Iss 8, p e0248381 (2021) PLoS ONE, Vol 16, Iss 8 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0248381 |
| Popis: | Biological phenomena induced by terahertz (THz) irradiation are described in recent reports, but underlying mechanisms, structural and dynamical change of specific molecules are still unclear. In this paper, we performed time-lapse morphological analysis of human cells and found that THz irradiation halts cell division at cytokinesis. At the end of cytokinesis, the contractile ring, which consists of filamentous actin (F-actin), needs to disappear; however, it remained for 1 hour under THz irradiation. Induction of the functional structures of F-actin was also observed in interphase cells. Similar phenomena were also observed under chemical treatment (jasplakinolide), indicating that THz irradiation assists actin polymerization. We previously reported that THz irradiation enhances the polymerization of purified actin in vitro; our current work shows that it increases cytoplasmic F-actin in vivo. Thus, we identified one of the key biomechanisms affected by THz waves. |
| Databáze: | OpenAIRE |
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