Production and characteristics of highly specific antibodies to aldosterone
| Autor: | J.D.H. Slater, E.R. Smith, T.P. Jowett |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
medicine.medical_specialty
Radioimmunoassay Cross Reactions Biochemistry chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Avidity Bovine serum albumin Aldosterone biology Serum Albumin Bovine Specific antibody chemistry Acetylation Antibody Formation biology.protein Primary immunization Immunization Rabbits Antibody |
| Zdroj: | Journal of Steroid Biochemistry. 6:1415-1419 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(75)90078-3 |
| Popis: | The 3-carboxymethoxime of aldosterone 18,21-diacetate has been prepared and after enzymic deacetylation the product has been covalently linked to bovine serum albumin. This immunogenic complex when injected into rabbits (6), produced antibodies of high specificity and avidity in all animals within 16 weeks, as assessed by radioimmunoassay. The importance of assessing specificity at several different levels of response is stressed. All antibodies showed a reduction of cross-reactivity as the response (% free) increased but there was great variability in the rate of change of cross-reactivity with the magnitude of the response. Specificity appeared to remain unchanged, or to fall slightly, 21 weeks after primary immunization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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