Planar asymmetric lipid bilayers of glycosphingolipid or lipopolysaccharide on one side and phospholipids on the other: membrane potential, porin function, and complement activation
| Autor: | K. Kawahara, U Zähringer, Andre Wiese, J.O. Reiners, Ulrich Seydel, Klaus Brandenburg |
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| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Sphingomonas paucimobilis Surface Properties Lipid Bilayers Molecular Sequence Data Biophysics Porins In Vitro Techniques Biophysical Phenomena Glycosphingolipids Membrane Potentials chemistry.chemical_compound Glycolipid Salmonella Gram-Negative Bacteria Moiety Humans Lipid bilayer Complement Activation Phospholipids Membrane potential biology Chemistry Glycosphingolipid biology.organism_classification Membrane Biochemistry Carbohydrate Sequence Porin lipids (amino acids peptides and proteins) Research Article |
| Zdroj: | Biophysical Journal. 70(1):321-329 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/s0006-3495(96)79573-5 |
| Popis: | We have determined some physicochemical properties of the monosaccharide-type fraction (GSL-1) of glycosphingolipids, the major glycolipid components of the outer leaflet of the Gram-negative species Sphingomonas paucimobilis. These properties included the state of order of the hydrocarbon moiety, the effective molecular area, surface charge density, and intrinsic transmembrane potential profile of reconstituted planar asymmetric GSL-1/phospholipid bilayer membranes. We have, furthermore, investigated the insertion into and the function of porin channels in the reconstituted bilayers and the complement-activating capability of GSL-1 surfaces. All results were compared with respective data for deep rough mutant lipopolysaccharide of Salmonella minnesota R595. We found a remarkable agreement in most functional properties of the two glycolipids. |
| Databáze: | OpenAIRE |
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