Transcriptome adaptation of the bovine mammary gland to diets rich in unsaturated fatty acids shows greater impact of linseed oil over safflower oil on gene expression and metabolic pathways
| Autor: | Nathalie Bissonnette, Chaouki Benchaar, Adolf A. Ammah, Eveline M. Ibeagha-Awemu, Pier-Luc Dudemaine, Ran Li, Xin Zhao |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
food.ingredient Linoleic acid Mammary gland Adaptation Biological PDK4 Biology Transcriptome 03 medical and health sciences chemistry.chemical_compound food Linseed oil Genetics Animals Humans Gene Regulatory Networks Food science Mammary Glands Human Fatty acid synthesis chemistry.chemical_classification Body Weight food and beverages High-Throughput Nucleotide Sequencing Reproducibility of Results Lipid metabolism Canadian Holstein cows Metabolism Lipid synthesis Animal Feed 030104 developmental biology chemistry Biochemistry Gene Expression Regulation Fatty Acids Unsaturated lipids (amino acids peptides and proteins) Cattle Metabolic Networks and Pathways Safflower oil Biotechnology Polyunsaturated fatty acid Research Article |
| Zdroj: | BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background Nutritional strategies can decrease saturated fatty acids (SFAs) and increase health beneficial fatty acids (FAs) in bovine milk. The pathways/genes involved in these processes are not properly defined. Next-generation RNA-sequencing was used to investigate the bovine mammary gland transcriptome following supplemental feeding with 5 % linseed oil (LSO) or 5 % safflower oil (SFO). Holstein cows in mid-lactation were fed a control diet for 28 days (control period) followed by supplementation with 5 % LSO (12 cows) or 5 % SFO (12 cows) for 28 days (treatment period). Milk and mammary gland biopsies were sampled on days-14 (control period), +7 and +28 (treatment period). Milk was used to measure fat(FP)/protein(PP) percentages and individual FAs while RNA was subjected to sequencing. Results Milk FP was decreased by 30.38 % (LSO) or 32.42 % (SFO) while PP was unaffected (LSO) or increased (SFO). Several beneficial FAs were increased by LSO (C18:1n11t, CLA:10t12c, CLA:9c11t, C20:3n3, C20:5n3, C22:5n3) and SFO (C18:1n11t, CLA:10t12c , C20:1c11, C20:2, C20:3n3) while several SFAs (C4:0, C6:0, C8:0, C14:0, C16:0, C17:0, C24:0) were decreased by both treatments (P |
| Databáze: | OpenAIRE |
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