Topical Application of Cyclosporine Reduces Epineurial Fibrosis: Gross Post-surgical, Histopathological and Ultrastructural Analysis in a Rat Sciatic Nerve Model
| Autor: | Ozgur Ismailoglu, Necdet Demir, Serdar Baki Albayrak, Nail Caglar Temiz, Nuri Eralp Cetinalp, Ilker Solmaz, Gamze Tanriover |
|---|---|
| Přispěvatelé: | Çukurova Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Pathology Extraneural Organ transplantation 03 medical and health sciences 0302 clinical medicine Fibrosis medicine Cerrahi business.industry Regeneration (biology) Sciatic nerve injury medicine.disease Sciatic Nerve Tacrolimus Surgery 030220 oncology & carcinogenesis Epineurial Fibrosis Ultrastructure Cyclosporine Rat Neurology (clinical) Sciatic nerve business 030217 neurology & neurosurgery |
| Popis: | AIm: To investigate the anti-scarring potential of topical cyclosporine on rat sciatic nerves. mATERIAl and mEThODS: Both sciatic nerves were exposed in 24 adult male albino Wistar rats, and an abrasion injury was made on the biceps femoris close to the sciatic nerve. Cotton pads soaked with cyclosporine (5 mg/mL) and saline (0.9% NaCl) were placed around the nerves for 10 minutes in the experimental group and control group, respectively. All rats were sacrificed 8 weeks later and the sciatic nerves were examined. Epineurial adhesions were assessed using light and electron microscopy. Quantitative histological parameters, epineurial thickness, and scar density were evaluated in the histological investigation. RESUlTS: Significantly fewer epineurial adhesions were observed in the cyclosporine group in the post-surgical assessment, and the histopathological and ultrastructural examinations of the nerve segments than in the controls. The cyclosporine-treated animals had a statistically significant reduction in the density and quantity of epineurial scarring compared with the controls. CONClUSION: Topical cyclosporine effectively reduces epineurial scar formation on rat sciatic nerves AIm: To investigate the anti-scarring potential of topical cyclosporine on rat sciatic nerves. mATERIAl and mEThODS: Both sciatic nerves were exposed in 24 adult male albino Wistar rats, and an abrasion injury was made on the biceps femoris close to the sciatic nerve. Cotton pads soaked with cyclosporine (5 mg/mL) and saline (0.9% NaCl) were placed around the nerves for 10 minutes in the experimental group and control group, respectively. All rats were sacrificed 8 weeks later and the sciatic nerves were examined. Epineurial adhesions were assessed using light and electron microscopy. Quantitative histological parameters, epineurial thickness, and scar density were evaluated in the histological investigation. RESUlTS: Significantly fewer epineurial adhesions were observed in the cyclosporine group in the post-surgical assessment, and the histopathological and ultrastructural examinations of the nerve segments than in the controls. The cyclosporine-treated animals had a statistically significant reduction in the density and quantity of epineurial scarring compared with the controls. CONClUSION: Topical cyclosporine effectively reduces epineurial scar formation on rat sciatic nerves |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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