Differences in the expression of Ly-6C on neutrophils and monocytes following PI-PLC hydrolysis and cellular activation
| Autor: | Sandy Kurk, Davin B. Jutila, Mark A. Jutila |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Neutrophils
Neutrophile Lymphocyte Immunology Complement C5a Mice SCID Biology Monocytes Neutrophil Activation Flow cytometry Interferon-gamma Mice Phosphoinositide Phospholipase C Western blot Antigen medicine Immunology and Allergy Animals Antigens Ly Mice Inbred BALB C medicine.diagnostic_test Phosphoric Diester Hydrolases Tumor Necrosis Factor-alpha Monocyte Hydrolysis Phosphatidylinositol Diacylglycerol-Lyase Flow Cytometry Molecular biology Recombinant Proteins Up-Regulation medicine.anatomical_structure Cell culture Tetradecanoylphorbol Acetate Electrophoresis Polyacrylamide Gel Cell activation |
| Zdroj: | Immunology letters. 41(1) |
| ISSN: | 0165-2478 |
| Popis: | All leukocytes, except some subsets of lymphocytes, express Ly-6C. However, virtually all of the regulation studies of this antigen have been done on lymphocytes only. Recently we showed that monocytes and neutrophils express 10-100 times the level of Ly-6C expressed by lymphoid cells. We compared Ly-6C expression on neutrophils and monocytes following short-term cell activation induced by C5a or phorbol esters or treatment with phosphatidylinositol-specific phospholipase-C (PI-PLC). Activation of the neutrophil led to a rapid ( < 30 min) increase in surface expression of Ly-6C, but similar treatment had little effect on or slightly diminished the expression of the monocyte molecule. Prolonged treatment of the monocyte with IFN-gamma (24 h) led to increased Ly-6C expression. Low doses of TNF-alpha inhibited the up-regulation of Ly-6C induced by IFN-gamma. Similar to what has been described for the lymphocyte, the monocyte form of Ly-6C was hydrolyzed by PI-PLC treatment within 30 min. In contrast, the neutrophil molecule was unaffected by PI-PLC for up to 2 h. SDS-PAGE Western blot analysis demonstrated that the neutrophil and monocyte molecules were in the same molecular weight range (14-18 kDa Mr). Our results clearly show differences in the regulation of Ly-6C expression on monocytes versus neutrophils. Thus, it is likely that the potential function of Ly-6C will be unique for each cell. |
| Databáze: | OpenAIRE |
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