Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake
| Autor: | Georgii A. Zavarzin, Christina Probian, Jens Harder, Friedrich Widdel, Tatyana N. Zhilina, Ramona Appel, Enrique Llobet Brossa |
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| Rok vydání: | 2004 |
| Předmět: |
Bacterial gliding
DNA Bacterial Asia Molecular Sequence Data Succinic Acid Cellobiose Biochemistry Microbiology Enrichment culture DNA Ribosomal chemistry.chemical_compound RNA Ribosomal 16S Genetics Bacteroides Food science Molecular Biology In Situ Hybridization Phylogeny Acetic Acid chemistry.chemical_classification biology Genes rRNA General Medicine Pigments Biological Sequence Analysis DNA Hydrogen-Ion Concentration biology.organism_classification Cytophaga chemistry Fermentation Propionate Carbohydrate Metabolism Propionates Water Microbiology Flavobacterium Bacteria |
| Zdroj: | Archives of microbiology. 182(2-3) |
| ISSN: | 0302-8933 |
| Popis: | Anaerobic saccharolytic bacteria thriving at high pH values were studied in a cellulose-degrading enrichment culture originating from the alkaline lake, Verkhneye Beloye (Central Asia). In situ hybridization of the enrichment culture with 16S rRNA-targeted probes revealed that abundant, long, thin, rod-shaped cells were related to Cytophaga. Bacteria of this type were isolated with cellobiose and five isolates were characterized. Isolates were thin, flexible, gliding rods. They formed a spherical cyst-like structure at one cell end during the late growth phase. The pH range for growth was 7.5-10.2, with an optimum around pH 8.5. Cultures produced a pinkish pigment tentatively identified as a carotenoid. Isolates did not degrade cellulose, indicating that they utilized soluble products formed by so far uncultured hydrolytic cellulose degraders. Besides cellobiose, the isolates utilized other carbohydrates, including xylose, maltose, xylan, starch, and pectin. The main organic fermentation products were propionate, acetate, and succinate. Oxygen, which was not used as electron acceptor, impaired growth. A representative isolate, strain Z-7010, with Marinilabilia salmonicolor as the closest relative, is described as a new genus and species, Alkaliflexus imshenetskii. This is the first cultivated alkaliphilic anaerobic member of the Cytophaga/ Flavobacterium/ Bacteroides phylum. |
| Databáze: | OpenAIRE |
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