Potential application of simple easy-to-use insertion-deletion (InDel) markers in citrus cultivar identification

Autor:	Takashi Mihara, Takahiro Noda, Yukio Nagano, Kaoru Daiou
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Citrus genotype cultivar identification food and beverages InDel marker Plant Science Computational biology Biology Note Simple (abstract algebra) Genotype Genetics Insertion deletion Identification (biology) Cultivar Indel Agronomy and Crop Science
Zdroj:	Breeding Science
ISSN:	1347-3735 1344-7610
Popis:	We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a method for cultivar identification using these 28 InDel markers to genotype 31 citrus cultivars. The results revealed that a minimum of 6 markers were required to identify individuals using the three-genotype classification method. Furthermore, we found that a simple method for distinguishing between two genotypes (homozygous and heterozygous) could be used to identify individuals using a minimum of 7 markers. Our findings provide a basis for the development of simple and rapid citrus cultivar identification methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3a489c3b79f69543d27b183af1192b9 http://europepmc.org/articles/PMC8784345 Zobrazit plný text záznamu Plný text ve formátu PDF