A Chinese hamster mutant cell line with a defect in the integral membrane protein CII-3 of complex II of the mitochondrial electron transport chain
| Autor: | Per-Johan Meijer, Frank G. Oostveen, Immo E. Scheffler, Harry C. Au |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Centromere Molecular Sequence Data Restriction Mapping medicine.disease_cause Transfection Biochemistry Polymerase Chain Reaction Chinese hamster Cell Line Electron Transport Cricetulus Multienzyme Complexes Complementary DNA Cricetinae medicine Coding region Animals Humans Amino Acid Sequence Cloning Molecular Molecular Biology Gene Integral membrane protein DNA Primers chemistry.chemical_classification Mutation biology Base Sequence Sequence Homology Amino Acid C-terminus Electron Transport Complex II Cell Membrane Chromosome Mapping Cell Biology biology.organism_classification Molecular biology Recombinant Proteins Amino acid Mitochondria Succinate Dehydrogenase chemistry Cattle Oxidoreductases |
| Zdroj: | The Journal of biological chemistry. 270(44) |
| ISSN: | 0021-9258 |
| Popis: | In this study, a respiration-deficient Chinese hamster cell line with a defect in succinate dehydrogenase activity is shown to result from a single base change in a codon in the coding sequence for the membrane anchor protein CII-3 (also referred to as QPs-1). A premature translation stop results in the truncation of 33 amino acids from the C terminus. Bovine cDNA encoding this peptide complements the mutation. There is about 82% identity between these two mammalian proteins. The gene for CII-3 was mapped on human chromosome 1, and because it is also found on minichromosomes characterized by our laboratory, we can localize it on the short arm within 1-2 megabases from the centromere. |
| Databáze: | OpenAIRE |
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