Screening of Variations in CD22 Gene in Children with B-Precursor Acute Lymphoblastic Leukemia
| Autor: | Mine Mumcuoglu, Nejat Akar, A. Emin Kürekçi, Deniz Aslar Oner, Dilara Fatma Akin, Kadir Sipahi, Ustun Ezer |
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| Přispěvatelé: | TOBB ETU, Faculty of Medicine, Department of Internal Medical Sciences, TOBB ETÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Akar, Mehmet Nejat, H-2949-2019 |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Signal-Transduction Negative Regulator Binding Survival Adolescent Turkey Sialic Acid Binding Ig-like Lectin 2 Biology 03 medical and health sciences Exon Lymphocyte Antigen Receptor 0302 clinical medicine Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Gene duplication medicine Humans Child Gene Genetics (clinical) Polymorphism Genetic Base Sequence CD22 Exon 12 Deletion Genetic Variation Infant Single-strand conformation polymorphism General Medicine Exons Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Molecular biology Introns Leukemia genomic DNA 030104 developmental biology medicine.anatomical_structure Child Preschool Mutation Female Bone marrow Therapy 030215 immunology |
| Zdroj: | Genetic testing and molecular biomarkers. 20(9) |
| ISSN: | 1945-0257 |
| Popis: | Background: CD22 is expressed on the surface of B-cell lineage cells from the early progenitor stage of pro-B cell until terminal differentiation to mature B cells. It plays a role in signal transduction and as a regulator of B-cell receptor signaling in B-cell development. Objectives: We aimed to screen exons 9-14 of the CD22 gene, which is a mutational hot spot region in B-precursor acute lymphoblastic leukemia (pre-B ALL) patients, to find possible genetic variants that could play role in the pathogenesis of pre-B ALL in Turkish children. Methods: This study included 109 Turkish children with pre-B ALL who were diagnosed at Losante Hospital for Children with Leukemia. Genomic DNA was extracted from both peripheral blood and bone marrow leukocytes. Gene amplification was performed with PCR, and all samples were screened for the variants by single strand conformation polymorphism. Samples showing band shifts were sequenced on an automated sequencer. Results: In our patient group a total of 9 variants were identified in the CD22 gene by sequencing: a novel variant in intron 10 (T2199G); a missense variant in exon 12; 5 intronic variants between exon 12 and intron 13; a novel intronic variant (C2424T); and a synonymous in exon 13. Thirteen of 109 children (11.9%) carried the T2199G novel intronic variant located in intron 10, and 17 of 109 children (15.6%) carried the C2424T novel intronic variant. Conclusion: Novel variants in the CD22 gene in children with pre-B ALL in Turkey that are not present, in the Human Gene Mutation Database or NCBI SNP database, were found. |
| Databáze: | OpenAIRE |
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