Effects of high and low inspired fractions of oxygen on horse erythrocyte membrane properties, blood viscosity and muscle oxygenation during anaesthesia

Autor: David Crouzier, Nicole Fellmann, Jean-Claude Debouzy, Nathalie Kirschvink, Pierre Lekeux, Karine Portier, J. Coudert, Michel Guichardant, Michel Prost
Přispěvatelé: VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Male
Erythrocytes
040301 veterinary sciences
[SDV]Life Sciences [q-bio]
Blood viscosity
chemistry.chemical_element
Anesthesia
General
Horse
Oxygen
0403 veterinary science
03 medical and health sciences
Oxygen Consumption
medicine
Animals
Vitamin E
Horses
Muscle
Skeletal
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Oxygen saturation (medicine)
Hyperoxia
0303 health sciences
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
General Veterinary
business.industry
Cell Membrane
Erythrocyte membrane fluidity
04 agricultural and veterinary sciences
Oxygenation
Blood Viscosity
Haemolysis
Isoflurane
chemistry
Muscle perfusion and oxygen saturation
Oxidative stress
Anesthesia
Arterial blood
Lipid Peroxidation
medicine.symptom
Reactive Oxygen Species
business
medicine.drug
Zdroj: Veterinary Anaesthesia and Analgesia
Veterinary Anaesthesia and Analgesia, Wiley, 2009, 36 (4), pp.287-298. ⟨10.1111/j.1467-2995.2009.00459.x⟩
ISSN: 1467-2995
1467-2987
DOI: 10.1111/j.1467-2995.2009.00459.x⟩
Popis: Objectives To evaluate whether a period of hyperoxia or after a period of hypoxia produced changes attributable to reactive oxygen species in anaesthetized horses. Study design Prospective randomized experimental study. Animals Six healthy (ASA I) geldings, aged 4.5–9.5 years and weighing 510–640 kg −1 . Methods After 30 minutes breathing air as carrier gas for isoflurane, horses were assigned randomly to breathe air as carrier gas (CG0.21) or oxygen as carrier gas (CG1.00) for a further 90 minutes. After an interval of 1 month each horse was re-anaesthetized with the other carrier gas for the 90 minute test period. Ventilation was controlled throughout anaesthesia. Arterial blood was sampled to measure gas tensions, lactate, cholesterol, vitamin E, 4-hydroxy-alkenals, 8-epi-PGF 2α , half haemolysis time, half erythrolysis time, and erythrocyte membrane fluidity. Muscle blood flow and oxygenation were evaluated by near infrared spectroscopy and coloured Doppler. Results After the first 30 minutes horses were hypoxemic. Subsequently the CG1.00 group became hyperoxaemic (PaO 2 ∼240 mmHg) whereas the CG0.21 group remained hypoxaemic (PaO 2 ∼60 mmHg) and had increased lactate concentration. No significant changes in vitamin E, 4-hydroxy-alkenals, or 8-epi-PGF 2α concentrations were detected. During the 90 minute test period the CG0.21 group had increased resistance to free-radical-mediated lysis in erythrocytes, whereas the CG1.00 group had slightly decreased resistance of whole blood to haemolysis. CG0.21 induced a progressive muscle deoxygenation whereas CG1.00 induced an increase in muscle oxygen saturation followed by progressive deoxygenation towards baseline. Conclusions and clinical relevance During isoflurane anaesthesia in horses, the hyperoxia induced by changing from air to oxygen induced minimal damage from reactive oxygen species. Using air as the carrier gas decreased skeletal muscle oxygenation compared with using oxygen.
Databáze: OpenAIRE
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