In Vitro Genotoxicity Assessment from the Glycyrrhiza New Variety Extract
| Autor: | Jong-Sik Jin, Jae-Ki Chang, Hyo-Jin An, Jeong Hoon Lee, Sa-Haeng Kang, Wonnam Kim, Ju-Ryoun Soh, Young-Jae Song, Yong-Deok Jeon, Jonghyun Lee, Dong-Gu Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
QH301-705.5 QC1-999 medicine.disease_cause Chromosome aberration bacterial reverse mutation test Ames test chemistry.chemical_compound In vivo chromosome aberration test medicine General Materials Science Glycyrrhiza Biology (General) Instrumentation QD1-999 Fluid Flow and Transfer Processes biology Traditional medicine Process Chemistry and Technology Physics genotoxicity General Engineering biology.organism_classification Engineering (General). Civil engineering (General) In vitro Computer Science Applications wongam micronucleus test Chemistry chemistry Micronucleus test Liquiritigenin TA1-2040 Genotoxicity |
| Zdroj: | Applied Sciences, Vol 11, Iss 10257, p 10257 (2021) Applied Sciences Volume 11 Issue 21 |
| ISSN: | 2076-3417 |
| Popis: | The various species that comprise the genus Glycyrrhiza (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of Glycyrrhiza, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects. |
| Databáze: | OpenAIRE |
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