Reversible pulmonary trunk banding: Myocardial vascular endothelial growth factor expression in young goats submitted to ventricular retraining
| Autor: | Petronio Generoso Thomaz, Tiago A Meniconi, Marcelo Biscegli Jatene, Eduardo Arrais Rocha, Renato S. Assad, Maria Cristina Donadio Abduch, Vera Demarchi Aiello, Luiz F. P. Moreira |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine Cell signaling Physiology Social Sciences Hemodynamics Blood Pressure Signal transduction 030204 cardiovascular system & hematology Cardiovascular Physiology Muscle hypertrophy Training (Education) chemistry.chemical_compound Endocrinology 0302 clinical medicine Sociology Ventricular hypertrophy Medicine and Health Sciences Medicine Mammals Multidisciplinary Goats Eukaryota Heart Ruminants Hematology VEGF signaling Up-Regulation Vascular endothelial growth factor medicine.anatomical_structure Vertebrates Aortic pressure Cardiology Anatomy Research Article Cell biology medicine.medical_specialty Cardiac Ventricles Systole Science Heart Ventricles Cardiomegaly Pulmonary Artery Education 03 medical and health sciences Growth Factors Internal medicine Animals Endocrine Physiology business.industry Myocardium Retraining Organisms Biology and Life Sciences medicine.disease 030104 developmental biology Blood pressure chemistry Ventricle Amniotes Cardiovascular Anatomy Angiogenesis business Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 2, p e0217732 (2020) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND:Ventricle retraining has been extensively studied by our laboratory. Previous studies have demonstrated that intermittent overload causes a more efficient ventricular hypertrophy. The adaptive mechanisms involved in the ventricle retraining are not completely established. This study assessed vascular endothelial growth factor (VEGF) expression in the ventricles of goats submitted to systolic overload. METHODS:Twenty-one young goats were divided into 3 groups (7 animals each): control, 96-hour continuous systolic overload, and intermittent systolic overload (four 12-hour periods of systolic overload paired with 12-hour resting period). During the 96-hour protocol, systolic overload was adjusted to achieve a right ventricular (RV) / aortic pressure ratio of 0.7. Hemodynamic evaluations were performed daily before and after systolic overload. Echocardiograms were obtained preoperatively and at protocol end to measure cardiac masses thickness. At study end, the animals were killed for morphologic evaluation and immunohistochemical assessment of VEGF expression. RESULTS:RV-trained groups developed hypertrophy of RV and septal masses, confirmed by increased weight and thickness, as expected. In the study groups, there was a small but significantly increased water content of the RV and septum compared with those in the control group (p |
| Databáze: | OpenAIRE |
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