Aged garlic extract and S-allylcysteine increase the GLUT3 and GCLC expression levels in cerebral ischemia
| Autor: | Mónica Espinoza-Rojo, Carlos D. Gómez, Alma Ortiz-Plata, Eugenia Flores-Alfaro, Penélope Aguilera, María Elena Chánez-Cárdenas, Felipe Nares López, Martha Eugenia Ruiz-Tachiquín |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Glutamate-Cysteine Ligase Glucose Transport Proteins Facilitative Ischemia Medicine (miscellaneous) Pharmacology Neuroprotection Antioxidants General Biochemistry Genetics and Molecular Biology Brain Ischemia Internal Medicine medicine Animals Pharmacology (medical) Cysteine Viability assay Rats Wistar Garlic Genetics (clinical) chemistry.chemical_classification Reactive oxygen species biology Plant Extracts Chemistry Glucose transporter medicine.disease Rats Neuroprotective Agents GCLC Reperfusion Injury Reviews and References (medical) biology.protein Reperfusion injury GLUT3 |
| Zdroj: | Advances in Clinical and Experimental Medicine. 28:1609-1614 |
| ISSN: | 1899-5276 |
| DOI: | 10.17219/acem/110328 |
| Popis: | Background During cerebral ischemia, energy restoration through the regulation of glucose transporters and antioxidant defense mechanisms is essential to maintain cell viability. Antioxidant therapy has been considered effective to attenuate brain damage; moreover, the regulation of transcription factors that positively regulate the expression of glucose transporters is associated with this therapy. Recently, it has been reported that the use of antioxidants such as S-allylcysteine (SAC), a component of aged garlic extract (AGE), improves survival in experimental models of cerebral ischemia. Objectives The aim of this study was to determine the effect of AGE and SAC on the level of mRNA expression of the main neuronal glucose transporter (GLUT3) and the glutamate cysteine ligase catalytic subunit (GCLC) in rats with transient focal cerebral ischemia. Material and methods Cerebral ischemia was induced in male Wistar rats by middle cerebral artery occlusion (MCAO) for 2 h. The animals were sacrificed after different reperfusion times (0-48 h). Animals injected with AGE (360 mg/kg, intraperitoneally (i.p.)) and SAC (300 mg/kg, i.p.) at the beginning of reperfusion were sacrificed after 2 h. The mRNA expression level was analyzed in the fronto-parietal cortex using quantitative polymerase chain reaction (qPCR). Results Two major increases in GLUT3 expression at 1 h and 24 h of reperfusion were found. Both treatments increased GLUT3 and GCLC mRNA levels in control and under ischemic/reperfusion injury animals. Conclusions This data suggests that SAC and AGE might induce neuroprotection, while controlling reactive oxygen species (ROS) levels, as indicated by the increase in GCLC expression, and regulating the energy content of the cell by increasing glucose transport mediated by GLUT3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|