Induction by glucose of an antimycin-insensitive, azide-sensitive respiration in the yeast Kluyveromyces lactis
| Autor: | A. Goffeau, Iliana Ferrero, Anna-Maria Viola |
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| Rok vydání: | 1981 |
| Předmět: |
inorganic chemicals
Azides Sodium Antimycin A chemistry.chemical_element Carbohydrate metabolism Microbiology Fungal Proteins chemistry.chemical_compound Oxygen Consumption Ascomycota Sodium Cyanide Respiration Cytochrome c oxidase heterocyclic compounds Sodium Azide Molecular Biology Kluyveromyces lactis biology organic chemicals fungi General Medicine biology.organism_classification Glucose chemistry Biochemistry Saccharomycetales biology.protein Carbohydrate Metabolism Sodium azide sense organs Azide |
| Zdroj: | Antonie van Leeuwenhoek. 47:11-24 |
| ISSN: | 1572-9699 0003-6072 |
| Popis: | Increasing the glucose concentration from 0.1 to 10% in exponentially growing cultures of Kluyveromyces lactis CBS 2359 does not repress the antimycin-sensitive respiration (QO2 of 80 microliter O2 . h-1 . mg-1 dry weight) but raises the antimycin-insensitive respiration from 3 to 12 microliter O2 . h-2 . mg-1 dry weight. Antimycin A inhibits the growth of K. lactis on a variety of substrates with the exception of glucose at concentrations equal to or higher than 1% where substantial antimycin-insensitive respiratory rates are induced. It can be concluded that a minimal antimycin-insensitive QO2 is necessary for cellular growth when the normal respiratory pathway is not functional. The antimycin-insensitive respiration elicited by growth in high glucose concentrations is poorly inhibited by hydroxamate and is inhibited by 50% by 90 microM azide or 1 mM cyanide. These concentrations are much higher than those necessary to inhibit cytochrome c oxidase which is not involved in the antimycin-insensitive respiration as was demonstrated by spectral measurements. A pigment absorbing at 555 nm is specifically reduced after addition of glucose to antimycin-inhibited cells. The same pigment is reoxidized by further addition of high concentrations of sodium azide indicating its participation in the antimycin-insensitive, azide-sensitive respiration. |
| Databáze: | OpenAIRE |
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