Raf-Induced Vascular Endothelial Growth Factor Augments Kaposi's Sarcoma-Associated Herpesvirus Infection
| Autor: | Khalief E. Hamden, Ossie F. Dyson, Shi Yuan Cheng, Audy G. Whitman, Shaw M. Akula, Patrick W. Ford, James A. McCubrey |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Vascular Endothelial Growth Factor A
Small interfering RNA medicine.medical_treatment Immunology Microbiology chemistry.chemical_compound Foreskin Virology medicine Humans Gammaherpesvirinae RNA Small Interfering Cells Cultured Vascular Endothelial Growth Factor Receptor-1 biology Growth factor Kinase insert domain receptor Transfection biology.organism_classification Vascular Endothelial Growth Factor Receptor-2 Molecular biology Virus-Cell Interactions Vascular endothelial growth factor Vascular endothelial growth factor A medicine.anatomical_structure chemistry Insect Science Herpesvirus 8 Human raf Kinases |
| Zdroj: | Journal of Virology. 78:13381-13390 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.78.23.13381-13390.2004 |
| Popis: | Recombinant green fluorescent protein encoding Kaposi's sarcoma-associated herpesvirus (rKSHV.152) infection of β-estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ΔB-Raf [FF] :ER (expressing a weaker form of B-Raf) could be enhanced to levels comparable to that of HFF/ΔB-Raf [DD] :ER cells by pretreating cells with soluble vascular endothelial growth factor (VEGF). Conversely, VEGF expression and infection efficiency typically observed in β-estradiol stimulated HFF/ΔB-Raf [DD] :ER cells could be lowered significantly by treating with VEGF small interfering RNA. In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF 121 . These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|