Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells
| Autor: | Yoshikazu Nishiguchi, Mayu Onozato, Yutaro Azuma, Kato Kiyomasa, Hideaki Ichiba, Ayako Shimizu, Hiroshi Yamada, Erina Taniguchi, Takeshi Fukushima |
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| Rok vydání: | 2015 |
| Předmět: |
Stereochemistry
Apoptosis Mass spectrometry Ferric Compounds Medicinal chemistry General Biochemistry Genetics and Molecular Biology Biomaterials Structure-Activity Relationship Tumor Cells Cultured medicine Humans Chelation skin and connective tissue diseases IC50 Etoposide Cell Proliferation Dose-Response Relationship Drug Molecular Structure Chemistry Ligand Hydrazones Metals and Alloys Hep G2 Cells Oxaliplatin Terminal deoxynucleotidyl transferase Benzaldehydes MCF-7 Cells General Agricultural and Biological Sciences human activities medicine.drug |
| Zdroj: | BioMetals. 28:669-677 |
| ISSN: | 1572-8773 0966-0844 |
| DOI: | 10.1007/s10534-015-9852-x |
| Popis: | We previously developed a chelating ligand, 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone (HMB-ASH), which can chelate Fe(III) to form a complex. The HMB-ASH-Fe(III) complex exhibits a dose-dependent anti-proliferative effect in HepG2 cells, whereas the ligand, HMB-ASH, and Fe(III) alone had no considerable effect. The HMB-ASH-Fe(III) complex was composed of Fe(III):HMB-ASH (1:2), as determined by high-performance liquid chromatography with high-resolution mass spectrometry. The IC50 value was approximately 20 μM, which was comparable to those of the anti-cancer drugs oxaliplatin (OXP) and etoposide (ETP) under the same conditions. Similar to OXP and ETP, HMB-ASH-Fe(III) induced apoptosis in HepG2 cells, as revealed by terminal deoxynucleotidyl transferase fluorescein-12-dUTP nick end labeling assay. |
| Databáze: | OpenAIRE |
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