Regulatory mechanism of chicken lysozyme gene expression in oviducts examined using transgenic technology
| Autor: | Yuya Okuzaki, Shinji Iijima, Hidenori Kaneoka, Yusuke Kojima, Seiya Asai, Ken-ichi Nishijima, Shuichiro Moriwaki |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine animal structures Transgene DNase I hypersensitive sites Gene Expression Bioengineering Oviducts Biology 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Gene expression Animals Deoxyribonuclease I Cloning Molecular Promoter Regions Genetic Enhancer Gene Promoter Oviduct Cell biology Chicken lysozyme Transgenic chicken 030104 developmental biology chemistry embryonic structures Female Muramidase DNase I hypersensitive site Lysozyme Chickens Biotechnology Egg white |
| Zdroj: | Journal of Bioscience and Bioengineering. 131:453-459 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2020.11.011 |
| Popis: | The use of promoters that strongly express target genes in the chicken oviduct is beneficial for the production of proteinaceous materials into egg white by transgenic chickens. To examine the regulatory mechanisms of chicken lysozyme gene expression in vivo, genetically manipulated chickens that express human erythropoietin under the control of a lysozyme promoter-enhancer were established. By using several deletion mutants of the promoter-flanking region, we found that a −1.9 kb DNase I hypersensitive site (DHS) was essential for oviduct-specific expression in genetically manipulated chickens. The concentration of human erythropoietin in egg white was 14–75 μg/ml, suggesting that the chicken lysozyme promoter containing −1.9 kb DHS is sufficient for the production of pharmaceuticals using transgenic chickens. |
| Databáze: | OpenAIRE |
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