Analysis of the Cytotoxic Potential of Anisomelic Acid Isolated from Anisomeles malabarica
| Autor: | Christo Paul Preethy, Mohammad Abdulkadher Akbarsha, Ali Abdullah Alshatwi, Muthukumaran Gunasekaran |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty DNA damage Cytotoxicity Pharmaceutical Science Apoptosis Anisomeles malabarica chemistry.chemical_compound medicine MTT assay Viability assay biology business.industry Anti-cancer Acridine orange Anisomelic acid biology.organism_classification Molecular biology Comet assay chemistry Ethidium bromide business Research Article |
| Zdroj: | Scientia Pharmaceutica Volume 81 Issue 2 Pages 559-566 |
| ISSN: | 2218-0532 |
| Popis: | Anisomelic acid (AA), one of the major compounds in Anisomeles malabarica, was tested for its cytotoxicity and apoptosis-inducing potential in breast and cervical cancer cells. The MTT assay for cell viability indicated that AA is cytotoxic to all of the four cell lines tested in a dose- and duration-dependent manner. Acridine Orange & Ethidium Bromide (AO & EB) and Hoechst 33258 staining of AA-treated cells revealed typical apoptotic morphology such as condensed chromatin and formation of apoptotic bodies. The comet assay revealed DNA strand break(s), indicating that AA induces DNA damage which culminates in apoptosis. Thus, the study revealed the anti-proliferative and apoptosis-inducing properties of AA in both breast and cervical cancer cells. Therefore, anisomelic acid offers potential for application in breast and cervical cancer therapy. |
| Databáze: | OpenAIRE |
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