RGD-directed attachment of isolated rat osteoclasts to osteopontin, bone sialoprotein, and fibronectin
| Autor: | Göran Andersson, Dick Heinegård, Myra E. Flores, Finn P. Reinholt, Maria Norgård |
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| Rok vydání: | 1992 |
| Předmět: |
musculoskeletal diseases
Bone sialoprotein medicine.medical_specialty Sialoglycoproteins Molecular Sequence Data Integrin Glycine Osteoclasts Arginine Bone tissue Antibodies Bone and Bones Extracellular matrix stomatognathic system Osteoclast Internal medicine Cell Adhesion medicine Integrin-Binding Sialoprotein Animals Amino Acid Sequence Osteopontin Cells Cultured Dose-Response Relationship Drug biology Rats Inbred Strains Cell Biology Fibronectins Rats Cell biology Fibronectin medicine.anatomical_structure Endocrinology biology.protein Asparagine Peptides |
| Zdroj: | Experimental Cell Research. 201:526-530 |
| ISSN: | 0014-4827 |
| Popis: | Osteoclasts isolated from the long bones of 5-day-old rats were seeded onto glass surfaces coated with osteopontin, bone sialoprotein, or fibronectin. Cell binding was promoted by all three proteins and inhibited in a dose-dependent manner by an RGD-containing peptide, while an RGE-containing peptide was ineffective. Immunocytochemistry of bone tissue showed enhanced concentration of osteopontin in bone opposite the clear zone of the osteoclasts, whereas immunolocalization of bone sialoprotein and fibronectin showed no accumulation on bone surfaces facing cells. The observations corroborate previous findings that the osteoclast is attached via an integrin to osteopontin on the bone surface. Although bone sialoprotein and fibronectin can mediate osteoclast binding in vitro, such a role in vivo is not supported by the immunocytochemical observations. |
| Databáze: | OpenAIRE |
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