Mink-mouse hybridomas that secrete mink immunoglobulin G
| Autor: | O. K. Baranov, N.L. Galakhar, S.N. Djatchenko, E.S. Belousov, Ludmila V. Mechetina, A. M. Nayakshin, I I Fomicheva, Alexander V. Taranin |
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| Rok vydání: | 1988 |
| Předmět: |
animal diseases
Immunoblotting Immunology Mice Nude Enzyme-Linked Immunosorbent Assay Spleen Immunoglobulin G Cell Line Cell Fusion Mice Immune system Species Specificity biology.animal medicine Animals Immunology and Allergy Mink Cloning Mice Inbred BALB C Hybridomas Cell fusion biology Virology Molecular biology medicine.anatomical_structure Cell culture biology.protein Antibody |
| Zdroj: | Journal of Immunological Methods. 115:39-43 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(88)90307-9 |
| Popis: | Optimum conditions were established to obtain mink-mouse interspecific hybridomas secreting mink IgG in fusions of mouse myelomas with mink immune spleen cells. Minks were immunized with allogeneic IgG, and the spleen cells were fused with three mouse myeloma lines P3-X63-Ag8.653, NSO and Sp2/0-Ag14. Of these, P3-X63-Ag8.653 and NSO were found to be the best fusion partners giving the highest yield of hybrid clones and number of IgG secreting clones. Cloning of mink-mouse hybridomas was efficient when BALB/c nu/nu peritoneal and spleen cells were used as feeders. The ten clonal lines produced secreted intact mink IgG molecules as shown by SDS-PAGE and subsequent immunoblotting. The secretion level of IgG ranged from 5 to 200 ng/ml in the clonal lines. |
| Databáze: | OpenAIRE |
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