Endotoxemia and circulating bacteriome in severe COVID-19 patients
| Autor: | Win Kulvichit, Sunchai Payungporn, Asada Leelahavanichkul, Sadudee Peerapornratana, John A. Kellum, Opass Putcharoen, Kanitha Tiankanon, Prapaporn Pisitkun, Sarinya Kampunya, Kearkiat Praditpornsilpa, Navaporn Worasilchai, Somkanya Tungsanga, Napplika Kongpolprom, Thammasak Thawitsri, Trairak Pisitkun, Nattachai Srisawat, Ariya Chindamporn, Usa Thisyakorn, Thitiwat Sriprasart, Nuttha Lumlertgul, Kriang Tungsanga, Somchai Eiam-Ong, Vorthon Sawaswong, Terapong Tantawichien, Vorakamol Phoophiboon, Rujipat Samransamruajkit, Suwalak Chitcharoen, Visith Sitprija, Chintana Chirathaworn, Phatadon Sirivongrangson, Nophol Leelayuwatanakul, Tueboon Sriphojanart |
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| Rok vydání: | 2020 |
| Předmět: |
Circulating bacteriome
medicine.medical_treatment Disease Critical Care and Intensive Care Medicine Sepsis Pathogenesis 03 medical and health sciences 0302 clinical medicine Intensive care Gene duplication medicine 030212 general & internal medicine Critically ill Research Articles 030304 developmental biology Mechanical ventilation 0303 health sciences Acute respiratory distress syndrome business.industry lcsh:Medical emergencies. Critical care. Intensive care. First aid COVID-19 Bacteriome lcsh:RC86-88.9 medicine.disease Endotoxemia Pathophysiology Pneumonia Immunology business |
| Zdroj: | Intensive Care Medicine Experimental, Vol 8, Iss 1, Pp 1-15 (2020) Intensive Care Medicine Experimental |
| Popis: | PurposeWhen severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary bacterial infection is uncommon. However, as epithelium are injured and barrier function is lost, bacterial products entering the circulation might contribute to the pathophysiology of COVID-19.MethodsWe studied 19 adults, severely ill patients with COVID-19 infection, who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March and 17th April 2020. Blood samples on day 1, 3, and 7 of enrollment were analyzed for endotoxin activity assay (EAA), (1→3)-β-D-Glucan (BG), and 16S rRNA gene sequencing to determine the circulating bacteriome.ResultsOf the 19 patients, 14 were in intensive care and 10 patients received mechanical ventilation. We found 8 patients with high EAA (≥ 0.6) and about half of the patients had high serum BG levels which tended to be higher in later in the illness. Although only 1 patient had a positive blood culture, 18 of 19 patients were positive for 16S rRNA gene amplification. Proteobacteria was the most abundant phylum. The diversity of bacterial genera was decreased overtime.ConclusionsBacterial DNA and toxins were discovered in virtual all severely ill COVID-19 pneumonia patients. This raises a previously unrecognized concern for significant contribution of bacterial products in the pathogenesis of this disease |
| Databáze: | OpenAIRE |
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