Expression of Toll-like receptor 9 in nose, peripheral blood and bone marrow during symptomatic allergic rhinitis
| Autor: | Rolf Uddman, Sven Björnsson, Mikael Benson, Mattias Fransson, Lennart Jansson, Lars-Olaf Cardell, Mikael Adner, Jonas S. Erjefält |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male Adult Pathology medicine.medical_specialty Adolescent Respiratory Medicine and Allergy metabolism [Seasonal] Mucous membrane of nose metabolism [Nasal Mucosa] metabolism [Leukocytes] Allergic Bone Marrow Leukocytes Medicine Humans Receptor Nose Rhinitis lcsh:RC705-779 business.industry Research Rhinitis Allergic Seasonal TLR9 lcsh:Diseases of the respiratory system Middle Aged metabolism [Toll-Like Receptor 9] Toll-Like Receptor 9 Nasal Mucosa medicine.anatomical_structure metabolism [Biological Markers] Immunology metabolism [Bone Marrow] Immunohistochemistry Female Nasal Lavage Fluid Bone marrow business Biomarkers |
| Zdroj: | Respiratory Research; 8(17), pp 17-17 (2007) Respiratory Research, Vol 8, Iss 1, p 17 (2007) Respiratory Research |
| ISSN: | 1465-9921 |
| Popis: | Background Allergic rhinitis is an inflammatory disease of the upper airway mucosa that also affects leukocytes in bone marrow and peripheral blood. Toll-like receptor 9 (TLR9) is a receptor for unmethylated CpG dinucleotides found in bacterial and viral DNA. The present study was designed to examine the expression of TLR9 in the nasal mucosa and in leukocytes derived from different cellular compartments during symptomatic allergic rhinitis. Methods The study was based on 32 patients with seasonal allergic rhinitis and 18 healthy subjects, serving as controls. Nasal biopsies were obtained before and after allergen challenge. Bone marrow, peripheral blood and nasal lavage fluid were sampled outside and during pollen season. The expression of TLR9 in tissues and cells was analyzed using immunohistochemistry and flow cytometry, respectively. Results TLR9 was found in several cell types in the nasal mucosa and in different leukocyte subpopulations derived from bone marrow, peripheral blood and nasal lavage fluid. The leukocyte expression was generally higher in bone marrow than in peripheral blood, and not affected by symptomatic allergic rhinitis. Conclusion The widespread expression of TLR9 in the nasal mucosa along with its rich representation in leukocytes in different compartments, demonstrate the possibility for cells involved in allergic airway inflammation to directly interact with bacterial and viral DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink