Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development
| Autor: | Jacqueline S. Penaloza, Ning Xie, Sunny Sun Kin Chan, Hannah R. Hagen, Matthew P Pappas |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Biophysics Biology Cell fate determination Muscle Development Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Basic Helix-Loop-Helix Transcription Factors medicine Animals RNA-Seq Progenitor cell Muscle Skeletal Molecular Biology Cells Cultured Myogenesis Mesenchymal stem cell Skeletal muscle Cell Differentiation Cell Biology Anti-Bacterial Agents Cell biology Haematopoiesis 030104 developmental biology medicine.anatomical_structure Doxycycline 030220 oncology & carcinogenesis Single-Cell Analysis Stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 520:284-290 |
| ISSN: | 0006-291X |
| Popis: | The Mesp1 lineage contributes to cardiac, hematopoietic and skeletal myogenic development. Interestingly, muscle stem cells residing in craniofacial skeletal muscles primarily arise from Mesp1+ progenitors, but those in trunk and limb skeletal muscles do not. To gain insights into the difference between the head and trunk/limb muscle developmental processes, we studied Mesp1+ skeletal myogenic derivatives via single-cell RNA-seq and other strategies. Using a doxycycline-inducible Mesp1-expressing mouse embryonic stem cell line, we found that the development of Mesp1-induced skeletal myogenic progenitors can be characterized by dynamic expression of PDGFRα and VCAM1. Single-cell RNA-seq analysis further revealed the heterogeneous nature of these Mesp1+ derivatives, spanning pluripotent and mesodermal to mesenchymal and skeletal myogenic. We subsequently reconstructed the single-cell trajectories of these subpopulations. Our data thereby provide a cell fate projection of Mesp1-induced skeletal myogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect