An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles

Autor: Fiona A. van Vollenstee, Michael S. Pepper, Barend Andre Stander, Sean Moolman, Lara Kotze, Marnie Potgieter, Andri Swanepoel, Annie M. Joubert, Karlien Kallmeyer
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Cell
lcsh:Medicine
Pathology and Laboratory Medicine
Biochemistry
030207 dermatology & venereal diseases
chemistry.chemical_compound
White Blood Cells
Mice
0302 clinical medicine
Animal Cells
Dermal Fillers
Medicine and Health Sciences
Adipocytes
Cell Cycle and Cell Division
Cytotoxicity
lcsh:Science
Immune Response
Connective Tissue Cells
Staining
Microscopy
Mice
Inbred BALB C
Multidisciplinary
Adipogenesis
Tissue Scaffolds
Cell Staining
Light Microscopy
Cell biology
medicine.anatomical_structure
Connective Tissue
Cell Processes
Polycaprolactone
Models
Animal
Female
Cellular Types
Anatomy
Research Article
Cell Survival
Immune Cells
Polyesters
Immunology
Primary Cell Culture
Research and Analysis Methods
03 medical and health sciences
Signs and Symptoms
In vivo
Diagnostic Medicine
medicine
Cell Adhesion
Animals
Viability assay
Cell Proliferation
Inflammation
Fibrin
Blood Cells
Tissue Engineering
Cell growth
lcsh:R
Biology and Life Sciences
Proteins
Cell Biology
Fibroblasts
Embryo
Mammalian
030104 developmental biology
Biological Tissue
chemistry
Cell culture
Specimen Preparation and Treatment
Polystyrenes
lcsh:Q
Zdroj: PLoS ONE, Vol 13, Iss 7, p e0198248 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: The field of dermal fillers is evolving rapidly and numerous products are currently on the market. Biodegradable polymers such as polycaprolactone (PCL) have been found to be compatible with several body tissues, and this makes them an ideal material for dermal filling purposes. Hollow PCL spheres were developed by the Council for Scientific and Industrial Research (CSIR) to serve both as an anchor point and a "tissue harbour" for cells. Particles were tested for cytotoxicity and cell adherence using mouse embryo fibroblasts (MEF). MEFs adhered to the particles and no significant toxic effects were observed based on morphology, cell growth, cell viability and cell cycle analysis, suggesting that the particles are suitable candidates for cell delivery systems in an in vivo setting. The objective of providing a "tissue harbour" was however not realized, as cells did not preferentially migrate into the ported particles. In vivo studies were conducted in BALB/c mice into whom particles were introduced at the level of the hypodermis. Mice injected with PCL particles (ported and non-ported; with or without MEFs) showed evidence of local inflammation and increased adipogenesis at the site of injection, as well as a systemic inflammatory response. These effects were also observed in mice that received apparently inert (polystyrene) particles. Ported PCL particles can therefore act as a cell delivery system and through their ability to induce adipogenesis, may also serve as a dermal bulking agent.
Databáze: OpenAIRE
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