Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast
Autor: | Theerayut Toojinda, Berlaine Quime, Apinya Longya, Siripar Korinsak, Mary Jeanie Telebanco-Yanoria, Siriporn Korinsak, Chaivarakun Chaipanya, Apichart Vanavichit, Chatchawan Jantasuriyarat, Bo Zhou |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Sequence analysis Soil Science Plant Science lcsh:Plant culture Biology 01 natural sciences 03 medical and health sciences Broad spectrum lcsh:SB1-1110 Allele Gene Durable resistance Genetics Oryza sativa Resistance (ecology) business.industry Quantitative trait loci (QTL) food and beverages Chromosome Magnaporthe oryzae Biotechnology 030104 developmental biology Original Article business Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Rice Rice, Vol 10, Iss 1, Pp 1-11 (2017) |
ISSN: | 1939-8433 1939-8425 |
DOI: | 10.1186/s12284-017-0159-0 |
Popis: | Background Rice (Oryza sativa) is one of the most important food crops in the world. Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. To effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. The Thai rice variety Jao Hom Nin (JHN) showed broad-spectrum resistance against Thai rice blast isolates. Two QTLs for blast resistance in JHN were reported on chromosome 1 (QTL1) and 11 (QTL11). Results Monogenic lines of QTL1 (QTL1-C) and QTL11 (QTL11-C) in the CO39 genetic background were generated. Cluster analysis based on the disease reaction pattern of QTL1-C and QTL11-C, together with IRBLs, showed that those two monogenic lines were clustered with IRBLsh-S (Pish) and IRBL7-M (Pi7), respectively. Moreover, sequence analysis revealed that Pish and Pi7 were embedded within the QTL1 and QTL11 delimited genomic intervals, respectively. This study thus concluded that QTL1 and QTL11 could encode alleles of Pish and Pi7, designated as Pish-J and Pi7-J, respectively. To validate this hypothesis, the genomic regions of Pish-J and Pi7-J were cloned and sequenced. Protein sequence comparison revealed that Pish-J and Pi7-J were identical to Pish and Pi7, respectively. The holistic disease spectrum of JHN was found to be exactly attributed to the additive ones of both QTL1-C and QTL11-C. Conclusion JHN showed broad spectrum resistance against Thai and Philippine rice blast isolates. As this study demonstrated, the combination of two resistance genes, Pish-J and Pi7-J, in JHN, with each controlling broad-spectrum resistance to rice blast disease, explains the high level of resistance. Thus, the combination of Pish and Pi7 can provide a practical scheme for breeding durable resistance in rice against rice blast disease. Electronic supplementary material The online version of this article (doi:10.1186/s12284-017-0159-0) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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