The Size and Phenotype of Virus-Specific T Cell Populations Is Determined by Repetitive Antigenic Stimulation and Environmental Cytokines
| Autor: | Ester B. M. Remmerswaal, S. Surachno, Ineke J. M. ten Berge, S. L. Yong, René A. W. van Lier, Pauline M. E. Wertheim-van Dillen, Laila E. Gamadia, Ester M. M. van Leeuwen |
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| Přispěvatelé: | Experimental Immunology, AII - Amsterdam institute for Infection and Immunity, Nephrology, Clinical Immunology and Rheumatology |
| Rok vydání: | 2004 |
| Předmět: |
Cytotoxicity
Immunologic T cell Immunology Dose-Response Relationship Immunologic Cytomegalovirus Epitopes T-Lymphocyte chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Biology Ligands Lymphocyte Activation CCL5 Immunophenotyping Viral Matrix Proteins Interleukin 21 Antigens CD T-Lymphocyte Subsets medicine Humans Immunology and Allergy Cytotoxic T cell Longitudinal Studies Lymphocyte Count IL-2 receptor Antigen-presenting cell Antigens Viral Cells Cultured ZAP70 Membrane Proteins virus diseases hemic and immune systems Phosphoproteins Natural killer T cell Molecular biology Tumor Necrosis Factor Receptor Superfamily Member 7 Virus Latency medicine.anatomical_structure Cytokines CD27 Ligand |
| Zdroj: | Journal of immunology (Baltimore, Md., 172(10), 6107-6114. American Association of Immunologists |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.172.10.6107 |
| Popis: | Based on the expression of the TNFR SFP CD27, two Ag-primed CD8+ T cell subsets can be discerned in the circulation of healthy individuals: CD27+ T cells that produce a variety of cytokines but do not display immediate cytolytic activity; and cytotoxic CD27− T cells, which secrete only IFN-γ and TNF-α. The mechanism that controls the generation of these different phenotypes is unknown. We show that CMV reactivation not only increases the number of virus-specific T cells but also induces their transition from a CD27+ to a CD27− phenotype. In support of a relation between pool size and phenotype in a cohort of latently infected individuals, the number of Ag-specific CD27− CD8+ T cells was found to be linearly related to the total number of CMV-specific CD8+ T cells. In vitro studies revealed that the acquisition of the CD27− phenotype on CMV-specific T cells depended on the interaction of CD27 with its cellular ligand, CD70. Expression of CD70 was proportional to the amount of antigenic stimulation and blocked by the CD4+ T cell-derived cytokine IL-21. Thus, induction of CD70, which may vary in distinct viral infections, appears to be a key factor in determining the size and phenotype of the CMV-specific T cell population in latently infected individuals. |
| Databáze: | OpenAIRE |
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