Adipose-derived stem cell sheet transplantation therapy in a porcine model of chronic heart failure
| Autor: | Noritoshi Nagaya, Hidezo Mori, Tatsuya Shimizu, Yoshiki Sawa, Osamu Ishida, Toshikatsu Yagihara, Ikuo Hagino, Teruo Okano |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Sus scrofa Neovascularization Physiologic Adipose tissue Ventricular Function Left Translational Research Biomedical Neovascularization Physiology (medical) Internal medicine medicine Animals Humans Circumflex Cell Proliferation Heart Failure Ejection fraction Tissue Engineering business.industry Multipotent Stem Cells Biochemistry (medical) Public Health Environmental and Occupational Health Cell Differentiation General Medicine medicine.disease Surgery Transplantation Disease Models Animal medicine.anatomical_structure Adipose Tissue Heart failure Cardiology Stem cell medicine.symptom business Artery |
| Zdroj: | Translational Research. 165:631-639 |
| ISSN: | 1931-5244 |
| DOI: | 10.1016/j.trsl.2014.12.005 |
| Popis: | Adipose-derived stem cells (ASCs) are a promising resource for cell transplantation therapy for damaged heart tissue. Cell death in the graft early after transplantation represents the main cause of unsatisfactory therapeutic efficacy, but tissue-engineered cell sheets grown in temperature-responsive cell culture dishes may enable improved engraftment of transplanted cells. We investigated the therapeutic potential of this method in chronic myocardial ischemia in swine. We created a porcine model of chronic heart failure by implanting an ameroid constrictor around the main trunk of the left anterior descending artery, just distal to the circumflex branch. Simultaneously, ASCs were obtained from a piece of subcutaneous adipose tissue and expanded to form ASC sheets using temperature-responsive dishes. Four weeks after ameroid constrictor placement, triple-layered ASC sheets were transplanted onto the area of the ischemic myocardium (sheet group, n = 7). Controls (n = 7) received no sheet. Just before and 4 weeks after transplantation, left ventriculography (LVG) and coronary angiography (CAG) were performed. LVG revealed a significant improvement in the left ventricular ejection fraction of the sheet group compared with controls (47.6 ± 2.9% vs 41.4 ± 2.8%, P |
| Databáze: | OpenAIRE |
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