Sequence-specific chemical modification of a 365-nucleotide-long DNA fragment with an alkylating oligonucleotide derivative
| Autor: | S.A. Grachev, Gorn Vv, Valentin V. Vlassov, S.A. Gaidamakov |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Mustard Compounds
Alkylation Chemical Phenomena Stereochemistry Oligonucleotides Biophysics Guanosine Sequence (biology) Biochemistry chemistry.chemical_compound Structural Biology Genetics Bacteriophages Nucleotide Cloning Molecular Molecular Biology Affinity modification chemistry.chemical_classification Base Sequence Oligonucleotide Chemical modification DNA Cell Biology Chemistry Oligodeoxyribonucleotides chemistry Nucleic acid Derivative (chemistry) Plasmids |
| Zdroj: | FEBS Letters. (2):415-418 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(85)80345-8 |
| Popis: | An aromatic 2-chloroethylamino group was attached to the 5'-terminal phosphate of the oligodeoxyribonucleotide pCCCTCTTTCTT. The oligonucleotide derivative prepared was used for modification of the 365-nucleotide-long DNA fragment. It was found that modification of the fragment proceeds in a sequence-specific way at 3 guanosine residues within the sequence complementary to the oligonucleotide reagent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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