Structural and chemical analysis of native and malted barley kernels by polarized Raman spectroscopy (PRS)
| Autor: | Tapani Vuorinen, Carlo G. Bertinetto, Ulla Holopainen, Leonardo Galvis, Tarja Tamminen |
|---|---|
| Přispěvatelé: | Department of Forest Products Technology, Aalto-yliopisto, Aalto University |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Chemical imaging
Starch education Analytical chemistry PRS Biochemistry Band-Target Entropy Minimization Spectral line LLS Endosperm Cell wall Ferulic acid chemistry.chemical_compound symbols.namesake Atomic force microscopy Linear least-squares fit ta216 Anisotropy ta215 SD Chemistry food and beverages Coefficient of Variation Crystallography BTEM Polarized Raman spectroscopy Multivariate analysis Standard Deviation symbols CV AFM Raman spectroscopy Malting Food Science |
| Zdroj: | Galvis, L, Bertinetto, C G, Holopainen, U, Tamminen, T & Vuorinen, T 2015, ' Structural and chemical analysis of native and malted barley kernels by polarized Raman spectroscopy (PRS) ', Journal of Cereal Science, vol. 62, pp. 73-80 . https://doi.org/10.1016/j.jcs.2014.12.008 |
| ISSN: | 1095-9963 0733-5210 |
| Popis: | In this work, barley kernel sections embedded in resin were mapped using polarized Raman spectroscopy (PRS) for evaluation of both chemical and structural changes in endosperm upon malting. A multivariate analysis that includes Band-Target Entropy Minimization (BTEM) was used to resolve the individual spectrum of components present on sections and to reconstruct images based on their relative signal. BTEM-resolved spectra matched with several individual model compound spectra that allowed the identification of protein, starch, non-starch carbohydrates, esterified ferulic acid and embedding media. Raman images also revealed chemical changes in the cell walls within starchy endosperm and inside cells due to enzymatic reactions in malting. The anisotropic response of the C–O–C Raman band associated with the orientation of ordered structures inside starch granules remains despite a notorious shape distortion of large starch granules. |
| Databáze: | OpenAIRE |
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