Identification and Clinical Significance of Helcococcus species, with Description of Helcococcus seattlensis sp. nov. from a Patient with Urosepsis
| Autor: | Jill E. Clarridge, Siu Kei Chow |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Microbiology (medical) medicine.drug_class Molecular Sequence Data Antibiotics Erythromycin Microbial Sensitivity Tests Gram-Positive Bacteria medicine.disease_cause DNA Ribosomal Microbiology Levofloxacin RNA Ribosomal 16S Sepsis medicine Humans Gram-Positive Bacterial Infections biology Clindamycin Bacteriology Sequence Analysis DNA Abiotrophia Helcococcus kunzii biology.organism_classification Anti-Bacterial Agents Bacterial Typing Techniques Blood Staphylococcus aureus Urinary Tract Infections Gentamicin medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 52:854-858 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.03076-13 |
| Popis: | Helcococcus spp. are Gram-positive, catalase-negative, facultatively anaerobic cocci that are associated with wound and prosthetic joint infections as well bacteremia and empyema. Five Helcococcus spp. strains were isolated from our patient population, including 2 strains of Helcococcus kunzii from trauma-associated wounds, 2 Helcococcus sueciensis strains from blood and abscess, and a novel Helcococcus spp. strain from blood associated with urosepsis. Based on the phenotypic and phylogenetic evidence, we propose that the unknown bacterium be classified as Helcococcus seattlensis sp. nov. We found that all 5 tested Helcococcus strains grew as satellite colonies around Staphylococcus aureus and, interestingly, both H. kunzii strains were isolated together with S. aureus . In addition to 16S rRNA gene sequencing, conventional methods for leucine aminopeptidase (LAP) and pyrrolidonyl arylamidase (PYR) testing can be cost-effective and efficient for differentiation of Helcococcus spp. from Abiotrophia and Granulicatella species. Using nonstandard methods, we found that all tested Helcococcus spp. had high MICs of >4/76 μg/ml for trimethoprim-sulfamethoxazole, an antibiotic commonly used to treat urinary tract infections. High MICs for erythromycin, azithromycin, and clindamycin, and intermediate to high MICs for moxifloxacin, levofloxacin, and gentamicin were also observed among the Helcococcus strains. |
| Databáze: | OpenAIRE |
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