Delta-6-desaturase gene polymorphism is associated with lipoprotein oxidation in vitro
| Autor: | Tiina Solakivi, Terho Lehtimäki, Seppo T. Nikkari, Olli Jaakkola, Tarja Kunnas, Jaana Renko |
|---|---|
| Přispěvatelé: | Biolääketieteellisen teknologian yksikkö - Institute of Biomedical Technology, Lääketieteen yksikkö - School of Medicine, University of Tampere |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Lipoproteins
LDL/blood Lipoprotein modification medicine.medical_specialty Lipid Peroxidation/genetics Genotype Biolääketieteet - Biomedicine FADS2 Lipoproteins Endocrinology Diabetes and Metabolism Clinical Biochemistry Biology In Vitro Techniques Linoleoyl-CoA Desaturase chemistry.chemical_compound Eicosapentaenoic Acid/blood Unsaturated/blood Endocrinology Internal medicine medicine Humans Arachidonic Acid/blood Lipoprotein oxidation Fatty Acids Unsaturated/blood Genetic Association Studies Sequence Deletion Biochemistry medical Arachidonic Acid Research Biochemistry (medical) Fatty Acids Linoleoyl-CoA Desaturase/genetics Eicosapentaenoic acid Delta-6-desaturase LDL/blood Lipoproteins LDL Fatty acid desaturase Eicosapentaenoic Acid Biochemistry chemistry Fatty Acids Unsaturated biology.protein lipids (amino acids peptides and proteins) Lipid Peroxidation Docosapentaenoic acid Linoleoyl-CoA desaturase Oxidation-Reduction |
| Zdroj: | Solakivi, T, Kunnas, T, Jaakkola, O, Renko, J, Lehtimäki, T & Nikkari, S T 2013, ' Delta-6-desaturase gene polymorphism is associated with lipoprotein oxidation in vitro ', Lipids in Health and Disease, vol. 12, 80 . https://doi.org/10.1186/1476-511X-12-80 Lipids in Health and Disease |
| DOI: | 10.1186/1476-511X-12-80 |
| Popis: | Background Oxidative modification of low-density lipoprotein (LDL) is a key event in the oxidation hypothesis of atherogenesis. We have previously shown that HDL does not protect LDL from oxidation in vitro, but is in fact oxidized fastest of all lipoproteins due to its rich polyunsaturated fatty acid (PUFA) composition, which is oxidation promoting. Evidence has accumulated to show that in addition to diet, common polymorphisms in the fatty acid desaturase (FADS) gene cluster have very marked effects on human PUFA status. There is a deletion [T/-] in the promoter region of the Δ6 –desaturase gene (FADS2, rs 3834458), which has a direct inhibitory influence on production of PUFA from linoleic and alpha-linolenic acid. To investigate the possible role of rs 3834458 in lipoprotein modification, oxidation of LDL with HDL2 or HDL3 were analyzed from plasma of 58 free-living individuals. Results Total eicosapentaenoic acid and arachidonic acid were significantly decreased in plasma from the 10 subjects homozygous for the deletion in FADS2 rs 3834458. When the isolated LDL and HDL2 were subjected to Cu2+-induced oxidation, these subjects showed decreased rate of appearance (p = 0.027) and the final concentration of conjugated dienes (p = 0.033) compared to the other genotypes. For oxidation of LDL with HDL3, the final concentration of conjugated dienes was also significantly decreased in subjects with [−/−] compared with [T/T] and [T/-] (p = 0.034). Conclusion We conclude that FADS2 genotype may play a role in peroxidation susceptibility of lipoproteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|