Chromosome painting and its applications in cultivated and wild rice
Autor: | Hengxiu Yu, Minghong Gu, Jiming Jiang, Meimei Yu, Weiyun Wang, Lili Hou, Wen Ji, Zhiyun Gong, Jianxiang Zhang, Tao Zhang, Zhihao Xu, Cenwen Zhu, Meng Xu |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Oryza eichingeri Chromosomal translocation Chromosome 9 Oryza sativa Plant Science Genome Chromosomes Plant Translocation Genetic Japonica 03 medical and health sciences Chromosome painting Chromosome (genetic algorithm) FISH Oligonucleotide lcsh:Botany In Situ Hybridization Fluorescence Chromosome Aberrations Whole genome sequencing Genetics biology food and beverages Oryza Aneuploidy biology.organism_classification lcsh:QK1-989 030104 developmental biology Chromosome variation Oligonucleotide Probes Genome Plant |
Zdroj: | BMC Plant Biology, Vol 18, Iss 1, Pp 1-10 (2018) |
ISSN: | 1471-2229 |
Popis: | Background The chromosome-specific probe is a fundamental tool of chromosome painting and has been commonly applied in mammalian species. The technology, however, has not been widely applied in plants due to a lack of methodologies for probe development. Identification and labeling of a large number of oligonucleotides (oligos) specific to a single chromosome offers us an opportunity to establish chromosome-specific probes in plants. However, never before has whole chromosome painting been performed in rice. Results We developed a pooled chromosome 9-specific probe in rice, which contains 25,000 oligos based on the genome sequence of a japonica rice (Oryza sativa L., AA, 2n = 2× = 24). Chromosome 9 was easily identified in both japonica and indica rice using this chromosome 9-painting probe. The probe was also successfully used to identify and characterize chromosome 9 in additional lines of O. sativa, a translocation line, two new aneuploids associated with chromosome 9 and a wild rice (Oryza eichingeri A. Peter, CC, 2n = 2× = 24). Conclusion The study reveals that a pool of oligos specific to a chromosome is a useful tool for chromosome painting in rice. |
Databáze: | OpenAIRE |
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