Epididymal expression of the forkhead transcription factor Foxi1 is required for male fertility
Autor: | Olle Söder, Sandra Rodrigo Blomqvist, Hilmar Vidarsson, Sven Enerbäck |
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Rok vydání: | 2006 |
Předmět: |
Male
Transcriptional Activation medicine.medical_specialty Vacuolar Proton-Translocating ATPases Carbonic anhydrase II Anion Transport Proteins Bicarbonate transporter protein Carbonic Anhydrase II General Biochemistry Genetics and Molecular Biology Article Mice Internal medicine Chlorocebus aethiops medicine Animals Promoter Regions Genetic Molecular Biology Regulation of gene expression Epididymis Reporter gene General Immunology and Microbiology biology General Neuroscience Forkhead Transcription Factors Pendrin Transfection Sperm Spermatozoa Cell biology Enzyme Activation Endocrinology medicine.anatomical_structure Fertility Gene Expression Regulation Sulfate Transporters COS Cells Mutation biology.protein Female |
Zdroj: | The EMBO journal. 25(17) |
ISSN: | 0261-4189 |
Popis: | An essential aspect of male reproductive capacity is the immediate availability of fertilization-ready spermatozoa. To ensure this, most mammals rely on post-testicular sperm maturation. In epididymis, germ cells are matured and stored in a quiescent state that readily can be altered to produce active spermatozoa. This depends on active proton secretion into the epididymal lumen. We have identified Foxi1 as an important regulator of gene expression in narrow and clear cells-the major proton secretory cells of epididymal epithelia. Foxi1 appears to be required for the expression of the B1-subunit of the vacuolar H+ -ATPase proton pump and for carbonic anhydrase II as well as the chloride/bicarbonate transporter pendrin. Using transfection experiments, we have identified a Foxi1 binding cis-element in the ATP6V1B1 (encoding the B1-subunit) promoter that is critical for reporter gene activation. When this site is mutated to eliminate Foxi1 binding, activation is also abolished. As a consequence of defect Foxi1-dependent epididymal sperm maturation, we demonstrate that spermatozoa from Foxi1 null males fail to reach the female genital tract in sufficient number to allow fertilization. |
Databáze: | OpenAIRE |
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