Malaria and Dengue Mosquito Vectors from Lao PDR Show a Lack of the rdl Mutant Allele Responsible for Cyclodiene Insecticide Resistance
| Autor: | Susannah Lechmere, Omobolanle H Abdullateef, Phoutmany Thammavong, Natalie M Portwood, Safina Khadam, Chloé Boer, Somsanith Chonephetsarath, Rosie Longstreeth, Paul T. Brey, Anna E Jacob, Nursu C Kuyucu, Wlaa Ali, Phonesavanh Luangamath, Jordan Forward, Andrew K. Jones, Santi Maithaviphet, Sébastien Marcombe, Somphat Nilaxay, Zuhal Rahmani, Simone Nambanya, Hayato Kakinuma, Natasha Brown, Joseph Hawkins, Penelope J Saverton, Madeleine Smee, Khaitong Lakeomany, Gabriela Stieger, Nothasin Phommavanh |
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| Přispěvatelé: | Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Oxford Brookes University, Ministry of Health [Laos], The mosquito collections, rearing and identification work was carried out within the framework of the MALVEC research project funded by the 5% initiative from the French Ministry of Foreign Affairs and International Development. For extraction and amplification of DNA from mosquitoes, funding was provided by Oxford Brookes University. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Insecticides
MESH: Dengue medicine.disease_cause Dengue fever Dengue chemistry.chemical_compound 0302 clinical medicine Aedes MESH: Insect Proteins MESH: Animals 0303 health sciences Mutation biology Anopheles dieldrin insecticide resistance MESH: Aedes 3. Good health Infectious Diseases Laos Insect Proteins MESH: Mosquito Vectors Aedes albopictus MESH: Mutation 030231 tropical medicine MESH: Malaria mosquito Aedes aegypti Mosquito Vectors MESH: Anopheles 03 medical and health sciences Dieldrin GABA receptor parasitic diseases medicine Animals MESH: Insecticide Resistance Alleles 030304 developmental biology General Veterinary MESH: Alleles fungi medicine.disease biology.organism_classification Virology Malaria MESH: Insecticides [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology chemistry MESH: Laos Insect Science MESH: Dieldrin Parasitology |
| Zdroj: | Journal of Medical Entomology Journal of Medical Entomology, Entomological Society of America, 2020, 57 (3), pp.815-823. ⟨10.1093/jme/tjz227⟩ |
| ISSN: | 0022-2585 |
| Popis: | The gamma-aminobutyric acid (GABA) receptor, RDL, plays important roles in neuronal signaling and is the target of highly effective insecticides. A mutation in RDL, commonly A296S, underlies resistance to several insecticides such as cyclodienes. Even though the use of cyclodienes has been banned, the occurrence of mutations substituting A296 is notably high in mosquitoes from several countries. Here, we report a survey investigating the prevalence of the Rdl mutant allele in mosquitoes from Laos, a country where mosquito-borne diseases such as malaria and dengue fever are health concerns. Anopheles and Aedes mosquitoes were collected from 12 provinces in Laos. Adult bioassays on Aedes aegypti (Linnaeus) (Diptera: Culicidae) and Aedes albopictus (Skuse) (Diptera: Culicidae) showed that all the populations tested were susceptible to dieldrin (4%) following WHO protocols. Exon 7 from a total of 791 mosquitoes was sequenced to identify the amino acid encoded for at 296 of RDL. Only one of these mosquitoes, Anopheles maculatus rampae Harbach and Somboon (Diptera: Culicidae) from Attapeu, carried the mutant allele being heterozygous for A296S. We therefore found a general lack of the Rdl mutant allele indicating that mosquitoes from Laos are not exposed to insecticides that act on the GABA receptor compared to mosquitoes in several other countries. Identifying the prevalence of the Rdl mutation may help inform the potential use of alternative insecticides that act on the GABA receptor should there be a need to replace pyrethroids in order to prevent/manage resistance. |
| Databáze: | OpenAIRE |
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