Serum miR-210 as a novel biomarker for molecular diagnosis of clear cell renal cell carcinoma
| Autor: | An Zhao, Marc Gigante, Christian Genin, Guorong Li, Michel Peoc'h |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Wilcoxon signed-rank test Clinical Biochemistry Real-Time Polymerase Chain Reaction Gastroenterology Pathology and Forensic Medicine Young Adult Internal medicine microRNA Biomarkers Tumor Carcinoma medicine Humans Carcinoma Renal Cell Molecular Biology Aged Aged 80 and over Receiver operating characteristic business.industry Middle Aged medicine.disease Kidney Neoplasms MicroRNAs Clear cell renal cell carcinoma Real-time polymerase chain reaction Molecular Diagnostic Techniques Mann–Whitney U test Biomarker (medicine) Female business |
| Zdroj: | Experimental and Molecular Pathology. 94:115-120 |
| ISSN: | 0014-4800 |
| Popis: | Our objective was to evaluate the levels of miR-210 in tumor and serum samples of conventional renal cell cancer (cRCC) patients to explore whether circulating miR-210 in serum can be used as a biomarker for the detection of cRCC.The paired samples from primary cRCC tumors and adjacent non-tumoral renal parenchyma were collected from 32 patients with cRCC. Serum samples were obtained from 68 patients with a cRCC before surgery, 10 samples after one week of surgery, and 42 healthy individuals were included in this study. Real-time PCR was used to measure the microRNA level. The expression of miRNAs was normalized using the dCT method. Expression levels of miR-210 were compared using the Mann-Whitney U test or Wilcoxon test. Diagnostic performance of serum miR-210 level was calculated by using the receiver operating characteristic (ROC) curve.The average miR-210 level was higher in primary cRCC tissues than in normal tissue (p=0.004). For serum samples, the average level of miR-210 was significantly higher in cRCC patients than in controls (p0.001). The serum miR-210 level yielded an AUC (the areas under the ROC curve) of 0.874 with a sensitivity of 81.0% and a specificity of 79.4%. Furthermore, the average serum level of miR-210 was significantly decreased in the patients one week after the operation (p=0.001).Serum mi-210 may have a potential as a novel noninvasive biomarker for the detection of cRCC. |
| Databáze: | OpenAIRE |
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