Long-Term Gene-Silencing Effects of siRNA Introduced by Single-Cell Electroporation into Postmitotic CNS Neurons
| Autor: | Masahiko Tanaka, Naohide Hirashima, Minami Asaoka, Yuchio Yanagawa |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Small interfering RNA
Cerebellum Green Fluorescent Proteins Cell Purkinje cell Biology Transfection Biochemistry Green fluorescent protein Mice Cellular and Molecular Neuroscience Golgi cell medicine Animals Humans Gene silencing Gene Knock-In Techniques Gene Silencing RNA Small Interfering Cells Cultured Neurons Electroporation General Medicine Molecular biology Mice Inbred C57BL medicine.anatomical_structure |
| Zdroj: | Neurochemical Research. 36:1482-1489 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-011-0474-6 |
| Popis: | To explore how long the gene-silencing effects of siRNA introduced into postmitotic neurons continue, we transferred siRNA against GFP into GFP-expressing Purkinje and Golgi cells in cerebellar cell cultures by single-cell electroporation. The temporal changes in the intensity of GFP fluorescence in the same electroporated cells were monitored in real time using GFP imaging. Under standard conditions, GFP fluorescence was reduced to under one-tenth of the initial levels 4-7 days after electroporation. Such effects continued at least up to 14 days after electroporation. The effects of siRNAs against endogenous genes also continued for the same period. Thus, this method could be an effective tool for silencing gene expression for a long period in postmitotic neurons. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink