Kinetic evaluation of lipophilic inhibitors of lipid peroxidation in DLPC liposomes
| Autor: | Alex R. Cazers, Barry S. Lutzke, John M. McCall, Kelly L. Horan, Dennis E. Epps |
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| Rok vydání: | 1994 |
| Předmět: |
Antioxidant
Free Radicals medicine.medical_treatment Linoleic acid Amidines Biochemistry Antioxidants Mass Spectrometry Piperazines Linoleic Acid Lipid peroxidation chemistry.chemical_compound Physiology (medical) Nitriles medicine Organic chemistry Reactivity (chemistry) Chromans Pregnatrienes Chromatography High Pressure Liquid Liposome Vesicle Substrate (chemistry) Free Radical Scavengers Combinatorial chemistry Kinetics Linoleic Acids chemistry Liposomes Phosphatidylcholines Radical initiator Lipid Peroxidation Azo Compounds |
| Zdroj: | Free Radical Biology and Medicine. 17:587-596 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/0891-5849(94)90098-1 |
| Popis: | The authors have developed a kinetic method that allows one to obtain relative reactivity constants for lipophilic antioxidants in free radical systems. Two experimental model systems were developed: (a) a methanolic solution using AMVN as the free radical initiator and linoleic acid as the substrate, and (b) a multilamellar vesicle system composed of dilinoleoylphosphatidylcholine and AAPH as the substrate and the initiator, respectively. The use of these two systems allows researchers not only to determine the intrinsic reactivity of a potential antioxidant, but also to evaluate its potency in a membranous system where the contribution of the physical properties of the antioxidant to the inhibition of lipid peroxidation is important. These results show that all antioxidants tested acted in these systems as free radical scavengers, and they validate the synergism between intrinsic scavenging ability and membrane affinity and/or membrane-modifying physical properties in the inhibition of lipid peroxidation. |
| Databáze: | OpenAIRE |
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