Modifying plant growth and development using the CDK inhibitor ICK1
| Autor: | Pete John, Hong Wang, Yongming Zhou, Ann L. Cleary, S. Whitwill, Lawrence C. Fowke, Susan Gilmer |
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| Rok vydání: | 2003 |
| Předmět: |
Microinjections
Arabidopsis Cell Cycle Proteins Brassica Plant Roots Cyclin-dependent kinase Arabidopsis thaliana Cyclin-Dependent Kinase Inhibitor Proteins Cyclin biology Arabidopsis Proteins Chemistry Kinase Cell Cycle fungi food and beverages Cell Biology General Medicine Cell cycle biology.organism_classification Cyclin-Dependent Kinases In vitro Cell biology Tradescantia Gene Targeting biology.protein Pollen CDK inhibitor |
| Zdroj: | Cell Biology International. 27:297-299 |
| ISSN: | 1065-6995 |
| Popis: | The cell cycle in plants, as in other eukaryotes, is regulated by cyclin-dependent protein kinases (CDKs), which are activated by association with cyclins and can be inhibited by the action of small protein inhibitors (review by Mironov et al., 1999). The first plant inhibitor of CDK, ICK1, was isolated from Arabidopsis thaliana (Wang et al., 1997, 1998). Although ICK1 shares sequence similarity over a short region with the mammalian p27 CDK inhibitor, ICK1 exhibits a unique structure and recombinant ICK1 specifically inhibits plant CDK activity by in vitro histone kinase assays. Three different approaches were used to study the effect of ICK1 in planta: (i) over-expression in Arabidopsis plants, (ii) targeted expression in Brassica plants and (iii) microinjection into Tradescantia stamen hair cells. |
| Databáze: | OpenAIRE |
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