Effect of a selective prostaglandin H synthase-2 inhibitor (NS-398) on prostaglandin production by the guinea-pig uterus
Autor: | N L Poyser, E. K. Naderali |
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Rok vydání: | 1996 |
Předmět: |
Embryology
medicine.medical_specialty Stromal cell Prostaglandins H Guinea Pigs Indomethacin Radioimmunoassay Uterus Prostaglandin Biology Endometrium Epithelium chemistry.chemical_compound Endocrinology Culture Techniques Internal medicine medicine Animals Cyclooxygenase Inhibitors Cells Cultured Nitrobenzenes reproductive and urinary physiology NS-398 Sulfonamides urogenital system Myometrium Obstetrics and Gynecology Cell Biology Molecular biology female genital diseases and pregnancy complications medicine.anatomical_structure Reproductive Medicine chemistry Prostaglandins Prostaglandin H2 Female lipids (amino acids peptides and proteins) |
Zdroj: | Reproduction. 108:75-80 |
ISSN: | 1741-7899 1470-1626 |
DOI: | 10.1530/jrf.0.1080075 |
Popis: | Using guinea-pig uterine tissues, indomethacin (a non-selective inhibitor of prostaglandin H synthase) inhibited prostaglandin (PG) synthesis by homogenates of the endometrium, by cultured endometrium and myometrium, and by cultured epithelial glandular cells and stromal cells derived from the endometrium. NS-398, a selective inhibitor of prostaglandin H synthase-2 (PGHS-2), also inhibited PG synthesis by endometrial homogenates, by cultured endometrium and myometrium, and by cultured epithelial glandular cells and stromal cells. Indomethacin and NS-398 inhibited PG production to similar extents, except for 6-keto-PGF1 alpha production by the myometrium where indomethacin was more effective. In particular, indomethacin and NS-398 produced over 90% inhibition of PGF2 alpha output from the epithelial glandular cells, the main source of PGF2 alpha in the endometrium. These functional studies indicate that prostaglandin H synthase-2 is the predominant PG-forming enzyme in the guinea-pig uterus. |
Databáze: | OpenAIRE |
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