Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
Autor: | Marek Plutinský, Jana Skřičková, Eva Pokojová, Petr Kubáň, Michal Greguš, Zdeněk Merta, Martina Doubková, Ilona Binková, Dagmar Kindlová, František Foret |
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Rok vydání: | 2015 |
Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Cystic Fibrosis Pulmonary Fibrosis Electrolyte Acetates 01 natural sciences Pulmonary Disease Chronic Obstructive 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Capillary electrophoresis Chlorides Sarcoidosis Pulmonary Bromide Crown Ethers Ammonium Compounds Humans Magnesium Exhaled breath condensate Lactic Acid Nitrite Nitrites Aged Ions Nitrates Chromatography Sulfates Sodium 010401 analytical chemistry Electrophoresis Capillary Repeatability Hydrogen-Ion Concentration Middle Aged Asthma respiratory tract diseases 0104 chemical sciences Butyrates Electrophoresis Breath Tests 030228 respiratory system chemistry Potassium Calcium Female Ethanesulfonic acid Propionates |
Zdroj: | Journal of Breath Research. 9:027107 |
ISSN: | 1752-7163 |
Popis: | The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD 1.3% (n = 7)) and peak areas (RSD 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics. |
Databáze: | OpenAIRE |
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