Myocardial oxygenation and functional recovery in infarct rat hearts transplanted with mesenchymal stem cells

Autor: Anna Bratasz, Brian K. Rivera, M. Lakshmi Kuppusamy, Simi M. Chacko, Ramasamy P. Pandian, Periannan Kuppusamy, Karuppaiyah Selvendiran, Mahmood Khan, Saradhadevi Varadharaj
Rok vydání: 2009
Předmět:
medicine.medical_specialty
Pathology
Time Factors
Physiology
medicine.medical_treatment
Cellular differentiation
Myocardial Infarction
Neovascularization
Physiologic
Mesenchymal Stem Cell Transplantation
Ventricular Function
Left
Oxygen Consumption
Fibrosis
Physiology (medical)
medicine
Animals
Regeneration
Transplantation
Homologous
Oximetry
Cells
Cultured
Cell Proliferation
business.industry
Regeneration (biology)
Myocardium
Mesenchymal stem cell
Electron Spin Resonance Spectroscopy
Cell Differentiation
Mesenchymal Stem Cells
Stroke Volume
Stem-cell therapy
Recovery of Function
medicine.disease
Myocardial Contraction
Endocytosis
Rats
Inbred F344
Surgery
Rats
Transplantation
Oxygen
Disease Models
Animal
Echocardiography
Molecular Probes
Circulatory system
Stem cell
Cardiology and Cardiovascular Medicine
business
Zdroj: American journal of physiology. Heart and circulatory physiology. 296(5)
ISSN: 0363-6135
Popis: Stem cell therapy for myocardial tissue repair is limited by the poor survival of transplanted cells, possibly because of inadequate supply of oxygen and nutrients. The purpose of this study was to assess the oxygenation level and functional recovery after allogenic transplantation of mesenchymal stem cells (MSC) in a rat model of myocardial infarction (MI). Myocardial oxygen tension (Po2) was measured by electron paramagnetic resonance oximetry using an implantable oxygen-sensing spin probe (OxySpin). MSCs incubated with OxySpins showed substantial uptake of the probe without affecting its oxygen sensitivity or calibration. The cells internalized with OxySpins were able to differentiate into osteogenic, adipogenic, cardiomyocyte, and endothelial cell lineages. The labeled cells tested positive for CD44 and CD29 markers and negative for the hematopoietic markers CD14 and CD45. For the in vivo studies, MI was induced in rats by permanently ligating the left anterior descending coronary artery. MSCs with OxySpins were transplanted in the infarct region of hearts. A significant increase in Po2 was observed in the MSC group compared with the untreated MI group (18.1 ± 2.6 vs. 13.0 ± 1.8 mmHg, n = 4, P < 0.05) at 4 wk after transplantation. Echocardiography showed a significant improvement in ejection fraction and fraction shortening, which inversely correlated with the magnitude of fibrosis in the treated hearts. The cell-transplanted hearts also showed an increase in vascular endothelial growth factor level and capillary density in the infarct region. The study established our ability to measure and correlate changes in myocardial tissue oxygenation with cardiac function in infarcted rat hearts treated with MSCs.
Databáze: OpenAIRE
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