Interaction of epigallocatechin-gallate and chlorhexidine with Streptococcus mutans stimulated odontoblast-like cells: Cytotoxicity, Interleukin-1β and co-species proteomic analyses
| Autor: | Anuradha Prakki, Céline M. Lévesque, Anil Kishen, Alexander Terry Stavroullakis, Lucelia Lemes Goncalves |
|---|---|
| Přispěvatelé: | University of Toronto, Universidade Estadual Paulista (UNESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Interleukin-1beta Epigallocatechin gallate Catechin Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Multiplicity of infection Tandem Mass Spectrometry medicine Cytotoxic T cell Animals Viability assay MDPC-23 LC-MS/MS Cytotoxicity General Dentistry 030304 developmental biology 0303 health sciences biology Odontoblasts Chemistry Chlorhexidine 030206 dentistry Cell Biology General Medicine biology.organism_classification Molecular biology Odontoblast Otorhinolaryngology IL-1β EGCG S. mutans medicine.drug Chromatography Liquid |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-29T08:33:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 Desert Research Institute Natural Sciences and Engineering Research Council of Canada Objectives: The dentin therapeutic agent chlorhexidine has inflammatory and cytotoxic characteristics urging investigation of alternatives like the natural compound epigallocatechin-gallate. The aim is to verify the effect of epigallocatechin-gallate and chlorhexidine on viability, interleukin-1β (IL-1β) and differential protein expression of MDPC-23 odontoblast-like cells stimulated by Streptococcus mutans. Design: Cells were stimulated with heat-killed S. mutans at multiplicity of infection (MOI) of 100–1000 and subsequently treated with 100–1 µM of epigallocatechin-gallate. Cells with no treatment or chlorhexidine were controls. Combined stimulated/treated cells were tested for cytotoxicity (Alamar-Blue, N = 3, n = 3), total protein (N = 3, n = 3), IL-1β (ELISA, N = 3, n = 3), and differential protein expression by liquid chromatography-tandem mass spectrometry (LC-MS/MS, n = 2). Results: Cells stimulated at MOI 100/1000 and treated with 10 µM epigallocatechin-gallate and chlorhexidine did not present cytotoxicity. IL-1β significantly increased in both un-stimulated and stimulated chlorhexidine 10 µM groups when compared to un-treated control (p < 0.05). MOI 100 chlorhexidine 10 µM group significantly increased IL-1β compared to un-stimulated chlorhexidine 10 µM and epigallocatechin-gallate 10 µM groups, as well as to MOI 100 epigallocatechin-gallate 10 µM group (p < 0.05). LC-MS/MS revealed S. mutans and mammalian proteins, with tooth-specific proteins exhibiting different abundance levels, depending on the tested condition. Conclusions: Odontoblast-like cells stimulated with S. mutans at different MOI combined with epigallocatechin-gallate treatment did not cause cytotoxicity. S. mutans stimulation combined with chlorhexidine 100 µM treatment decreased cell viability, while treatment with chlorhexidine 10 µM concentration significantly increased IL-1β. S. mutans stimulation and treatment of cells resulted in varied protein expression. Department of Clinical Sciences – Restorative Faculty of Dentistry University of Toronto Department of Restorative Dentistry Institute of Science and Technology of São José dos Campos Sao Paulo State University Department of Biological and Diagnostic Sciences-Oral Microbiology Faculty of Dentistry University of Toronto Dental Research Institute Faculty of Dentistry University of Toronto Department of Restorative Dentistry Institute of Science and Technology of São José dos Campos Sao Paulo State University Natural Sciences and Engineering Research Council of Canada: 2018-06489 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|