Human complement factor H and factor H-like protein 1 are expressed in human retinal pigment epithelial cells
| Autor: | Cordula Eddahabi, Christine Skerka, Andreas W. A. Weinberger, Dörthe Carstesen, Peter Walter, Peter F. Zipfel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
genetic structures
Complement C3b Inactivator Proteins/metabolism Regulator Interleukin-6/pharmacology Retinal Pigment Epithelium Complement Factor H/metabolism Cell Line Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Complement C3b Inactivator Proteins Humans Gene In Situ Hybridization Genetics Retinal pigment epithelium Dose-Response Relationship Drug Interleukin-6 Retinal General Medicine Immunohistochemistry eye diseases Sensory Systems Tissue Donors Retinal Pigment Epithelium/drug effects/metabolism Cell biology Complement system Ophthalmology medicine.anatomical_structure chemistry Microscopy Fluorescence Cell culture Factor H Complement Factor H Alternative complement pathway sense organs |
| Zdroj: | Ophthalmic Research, Vol. 51, No 2 (2014) pp. 59-66 |
| ISSN: | 1423-0259 0030-3747 |
| Popis: | Background: A common haplotype in the gene for the regulator of the alternative pathway of complement activation factor H has been linked to individual predisposition to age- related macular degeneration (AMD). Methods: In this study, retinal pigment epithelial (RPE) cells, i.e. immortalized ARPE-19 as well as primary human RPE cells, were investigated for expression of factor H and FHL-1 by immunohistochemistry and in situ hybridization analysis. Results: Factor H and the alternative spliced product FHL-1 are expressed in RPE cells, i.e. in immortalized ARPE-19 and primary human RPE cells. Factor H and FHL-1 expression was induced in a dose-dependent manner in ARPE-19 cells upon treatment with the inflammatory marker interleukin-6 (IL-6). In situ hybridization experiments confirmed an elevated expression rate of the factor H gene in IL-6-treated ARPE-19 cells. AMD is characterized by complement-associated inflammatory processes in the retina. Thus, local synthesis of complement regulators affects the protection of retinal cells and may be involved in the pathogenesis at the RPE-choroid interface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|